Proteintech

PD-1/CD279 Monoclonal Antibody (4H4D1)

PD-1/CD279 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PD-1/CD279 Antibody (66220-1-IG) in IHC (P)

Immunohistochemistry of paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue slide using 66220-1-Ig (PD-1/CD279 Antibody) at dilution of 1:200 (under 40x lens). . Heat mediated antigen retrieved with Tris-EDTA buffer, pH 9.0.

PD-1/CD279 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

PD-1/CD279 Antibody in Western Blot (WB)

PD-1/CD279 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

66220-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:4,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 8 publications 8 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

4H4D1

Immunogen

PD-1/CD279 Fusion Protein Ag12470 (1-288 aa encoded by BC074740)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.21 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MQIPQAPWP VVWAVLQLGW RPGWFLDSPD RPWNPPTFSP ALLVVTEGDN ATFTCSFSNT SESFVLNWYR MSPSNQTDKL AAFPEDRSQP GQDCRFRVTQ LPNGRDFHMS VVRARRNDSG TYLCGAISLA PKAQIKESLR AELRVTERRA EVPTAHPSPS PRPAGQFQTL VVGVVGGLLG SLVLLVWVLA VICSRAARGT IGARRTGQPL KEDPSAVPVF SVDYGELDFQ WREKTPEPPV PCVPEQTEYA TIVFPSGMGT SSPARRGSAD GPRSAQPLRP EDGHCSWPL (1-288 aa encoded by BC074740 )

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Fri May 19 00:00:00 UTC 2023

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Fri May 19 00:00:00 UTC 2023

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Fri May 19 00:00:00 UTC 2023

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Fri May 19 00:00:00 UTC 2023

Warming Menstruation and Analgesic Soup Inhibits PD-1/PD-L1 Pathway in Rats with Endometriosis.

Alternative therapies in health and medicine

Feng D,Wang X,Huang Y,Chen X,Xu R,Qu Z,Chou D

Mon May 01 00:00:00 UTC 2023

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT.

Biochemical pharmacology

Chang X,Liu J,Yang Q,Gao Y,Ding X,Zhao J,Li Y,Liu Z,Li Z,Wu Y,Zuo D

Thu Jun 01 00:00:00 UTC 2023

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT.

Biochemical pharmacology

Chang X,Liu J,Yang Q,Gao Y,Ding X,Zhao J,Li Y,Liu Z,Li Z,Wu Y,Zuo D

Thu Jun 01 00:00:00 UTC 2023

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT.

Biochemical pharmacology

Chang X,Liu J,Yang Q,Gao Y,Ding X,Zhao J,Li Y,Liu Z,Li Z,Wu Y,Zuo D

Thu Jun 01 00:00:00 UTC 2023

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT.

Biochemical pharmacology

Chang X,Liu J,Yang Q,Gao Y,Ding X,Zhao J,Li Y,Liu Z,Li Z,Wu Y,Zuo D

Thu Jun 01 00:00:00 UTC 2023

Electroacupuncture Alleviates CFA-Induced Inflammatory Pain via PD-L1/PD-1-SHP-1 Pathway.

Molecular neurobiology

Deng D,Xu F,Ma L,Zhang T,Wang Y,Huang S,Zhao W,Chen X

Mon May 01 00:00:00 UTC 2023

Anemoside B4 protects against chronic relapsing colitis in mice by modulating inflammatory response, colonic transcriptome and the gut microbiota.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Han Q,Deng LR,Zou M,Tang HZ,Huang CY,Chen FJ,Tomlinson B,Li YH

Tue Nov 01 00:00:00 UTC 2022

Overexpression of Programmed Cell Death 1 Prevents Doxorubicin-Induced Apoptosis Through Autophagy Induction in H9c2 Cardiomyocytes.

Cardiovascular toxicology

Kanno SI,Hara A

Sun May 01 00:00:00 UTC 2022

Overexpression of Programmed Cell Death 1 Prevents Doxorubicin-Induced Apoptosis Through Autophagy Induction in H9c2 Cardiomyocytes.

Cardiovascular toxicology

Kanno SI,Hara A

Sun May 01 00:00:00 UTC 2022

PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer.

Journal of materials chemistry. B

Zhu L,Liu JL,Yang JT,Wu DW,Xu N,Huo KF,Wang HB

Wed Nov 16 00:00:00 UTC 2022

PD-1 engineered cytomembrane cloaked molybdenum nitride for synergistic photothermal and enhanced immunotherapy of breast cancer.

Journal of materials chemistry. B

Zhu L,Liu JL,Yang JT,Wu DW,Xu N,Huo KF,Wang HB

Wed Nov 16 00:00:00 UTC 2022

Glutamine deprivation impairs function of infiltrating CD8⁺ T cells in hepatocellular carcinoma by inducing mitochondrial damage and apoptosis.

World journal of gastrointestinal oncology

Wang W,Guo MN,Li N,Pang DQ,Wu JH

Wed Jun 15 00:00:00 UTC 2022

Identification and experimental validation of a tumor-infiltrating lymphocytes-related long noncoding RNA signature for prognosis of clear cell renal cell carcinoma.

Frontiers in immunology

Deng Y,Guo K,Tang Z,Feng Y,Cai S,Ye J,Xi Y,Li J,Liu R,Cai C,Tan Z,Zhang Y,Han Z,Zeng G,Zhong W

Fri Dec 16 00:00:00 UTC 2022

Transcriptomic analysis of graft liver provides insight into the immune response of rat liver transplantation.

Frontiers in immunology

Cao W,Lu J,Li S,Song F,Xu J

Mon Nov 28 00:00:00 UTC 2022

CMTM6: increased circulating level and up-regulated expression in labial salivary glands in patients with primary Sjogren's syndrome.

Clinical and experimental immunology

Qian S,Xu J,Zhao S,Yang P,Yang C

Fri Jan 28 00:00:00 UTC 2022

Interaction of immune checkpoint PD-1 and chemokine receptor 4 (CXCR4) promotes a malignant phenotype in pancreatic cancer cells.

PloS one

Harper MM,Lin M,Cavnar MJ,Pandalai PK,Patel RA,Gao M,Kim J

Mon Jul 11 00:00:00 UTC 2022

The immunomodulatory effect of microglia on ECM neuroinflammation via the PD-1/PD-L1 pathway.

CNS neuroscience & therapeutics

Shen Y,Li Y,Zhu Q,Wang J,Huang Y,Liang J,Wu X,Zhao Y

Sat Jan 01 00:00:00 UTC 2022

The immunomodulatory effect of microglia on ECM neuroinflammation via the PD-1/PD-L1 pathway.

CNS neuroscience & therapeutics

Shen Y,Li Y,Zhu Q,Wang J,Huang Y,Liang J,Wu X,Zhao Y

Sat Jan 01 00:00:00 UTC 2022

Anemoside B4 ameliorates experimental autoimmune encephalomyelitis in mice by modulating inflammatory responses and the gut microbiota.

European journal of pharmacology

Zou M,Chen FJ,Deng LR,Han Q,Huang CY,Shen SS,Tomlinson B,Li YH

Thu Sep 15 00:00:00 UTC 2022

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Cancers

Nasarre P,Garcia DI,Siegel JB,Bonilla IV,Mukherjee R,Hilliard E,Chakraborty P,Nasarre C,Yustein JT,Lang M,Jaffa AA,Mehrotra S,Klauber-DeMore N

Sun May 30 00:00:00 UTC 2021

Targeted elimination of myeloid-derived suppressor cells via regulation of the STAT pathway alleviates tumor immunosuppression in neuroblastoma.

Immunology letters

Xu W,Li S,Li M,Yang X,Xie S,Lin L,Li G,Zhou H

Wed Dec 01 00:00:00 UTC 2021

The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin‑induced cardiotoxicity.

Molecular medicine reports

Kanno SI,Hara A

Mon Feb 01 00:00:00 UTC 2021

Elevated lymphocyte specific protein 1 expression is involved in the regulation of leukocyte migration and immunosuppressive microenvironment in glioblastoma.

Aging

Cao JY,Guo Q,Guan GF,Zhu C,Zou CY,Zhang LY,Cheng W,Wang GL,Cheng P,Wu AH,Li GY

Wed Jan 29 00:00:00 UTC 2020

Survival analysis with regard to PD-L1 and CD155 expression in human small cell lung cancer and a comparison with associated receptors.

Oncology letters

Xu Y,Cui G,Jiang Z,Li N,Zhang X

Fri Mar 01 00:00:00 UTC 2019

m⁶A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade.

Nature communications

Yang S,Wei J,Cui YH,Park G,Shah P,Deng Y,Aplin AE,Lu Z,Hwang S,He C,He YY

Tue Jun 25 00:00:00 UTC 2019

Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.

Frontiers in pharmacology

Zhang S,Zhou L,Zhang M,Wang Y,Wang M,Du J,Gu W,Kui F,Li J,Geng S,Du G

Fri Dec 22 00:00:00 UTC 2023

Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.

Frontiers in pharmacology

Zhang S,Zhou L,Zhang M,Wang Y,Wang M,Du J,Gu W,Kui F,Li J,Geng S,Du G

Fri Dec 22 00:00:00 UTC 2023

Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.

Frontiers in pharmacology

Zhang S,Zhou L,Zhang M,Wang Y,Wang M,Du J,Gu W,Kui F,Li J,Geng S,Du G

Fri Dec 22 00:00:00 UTC 2023

Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.

Frontiers in pharmacology

Zhang S,Zhou L,Zhang M,Wang Y,Wang M,Du J,Gu W,Kui F,Li J,Geng S,Du G

Fri Dec 22 00:00:00 UTC 2023

Programmed cell death ligand 1 (PD-L1) expression is not a predominant feature in Ewing sarcomas.

Pediatric blood & cancer

Spurny C,Kailayangiri S,Jamitzky S,Altvater B,Wardelmann E,Dirksen U,Hardes J,Hartmann W,Rossig C

Mon Jan 01 00:00:00 UTC 2018

Impact of mismatch-repair deficiency on the colorectal cancer immune microenvironment.

Oncotarget

Zhang Y,Sun Z,Mao X,Wu H,Luo F,Wu X,Zhou L,Qin J,Zhao L,Bai C

Tue Oct 17 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; programmed cell death 1 protein; Programmed cell death protein 1; programmed cell death protein 1-like; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2

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Gene Aliases: CD279; hPD-1; hPD-l; hSLE1; Ly101; PD-1; PD1; Pdc1; PDCD1; SLEB2

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UniProt ID: (Human) Q15116, (Mouse) Q02242

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Entrez Gene ID: (Human) 5133, (Rat) 301626, (Mouse) 18566

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Function(s)

signal transducer activity protein binding molecular_function

Process(es)

negative regulation of tolerance induction apoptotic process humoral immune response signal transduction multicellular organism development T cell costimulation negative regulation of apoptotic process positive regulation of T cell apoptotic process biological_process positive regulation of apoptotic process immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PD-1/CD279 Monoclonal Antibody (4H4D1)

PD-1/CD279 Antibody (66220-1-IG) in IHC (P)

Product Details

66220-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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