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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PD-L1 (CD274) Antibodies

          Proteintech

          PD-L1/CD274 Monoclonal Antibody (2B11D11)

          262 References
          View all (227) PD-L1 (CD274) antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite PD-L1/CD274 Monoclonal Antibody (2B11D11)

          • Antibody Testing Data (24)
          PD-L1/CD274 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          PD-L1/CD274 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 24

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          PD-L1/CD274 Antibody (66248-1-IG) in ICC/IF

          Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 66248-1-IG (PD-L1/CD274 antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PD-L1/CD274 Antibody in Immunocytochemistry (ICC/IF)
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Western Blot (WB)
          PD-L1/CD274 Antibody in Flow Cytometry (Flow)
          PD-L1/CD274 Antibody in Flow Cytometry (Flow)

          Product Details

          66248-1-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          View 125 publications 125 publications

          Immunohistochemistry (IHC)

          -
          View 87 publications 87 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:500-1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 42 publications 42 publications

          Flow Cytometry (Flow)

          0.2 µg/1x10^6 cells
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          -
          View 2 publications 2 publications

          Not applicable (N/A)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Pig, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2B11D11

          Immunogen

          PD-L1/CD274 Fusion Protein Ag12443 (25-241 aa encoded by BC074984)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite Plus 488
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1.55 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: KDLYVVEYG SNMTIECKFP VEKQLDLAAL IVYWEMEDKN IIQFVHGEED LKVQHSSYRQ RARLLKDQLS LGNAALQITD VKLQDAGVYR CMISYGGADY KRITVKVNAP YNKINQRILV VDPVTSEHEL TCQAEGYPKA EVIWTSSDHQ VLSGKTTTTN SKREEKLFNV TSTLRINTTT NEIFYCTFRR LDPEENHTAE LVIPELPLAH PPNERTHL (25-241 aa encoded by BC074984 )

          Target Information

          Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

          View more View less

          Gene Aliases: A530045L16Rik; B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1; RGD1566211

          View more View less

          UniProt ID: (Human) Q9NZQ7, (Mouse) Q9EP73

          View more View less

          Entrez Gene ID: (Pig) 574058, (Human) 29126, (Mouse) 60533, (Rat) 499342

          View more View less

          Function(s)
          protein binding
          Process(es)
          immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production response to cytokine positive regulation of T cell proliferation regulation of activated T cell proliferation negative regulation of activated T cell proliferation regulation of T cell apoptotic process toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion negative regulation of T cell proliferation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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