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Invitrogen
PDCD4 Recombinant Rabbit Monoclonal Antibody (JA80-83)
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FIGURE: 1 / 7
PDCD4 Antibody (MA5-42473) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of PDCD4 in PC-3M cells (green) using PDCD4 Recombinant Monoclonal Antibody (Product # MA5-42473). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-42473
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JA80-83
Immunogen
Synthetic peptide within human pdcd4 aa 425-469
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911614
Product Specific Information
Positive Control: A431, PC-3M, Hmuan tonsil tissue, human prostate tissue, human breasr cancer tissue, mouse colon tissue, MCF-7.
Subcellular Location: Nucleus. Cytoplasm.
Target Information
Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression was found to be upregulated in all cell lines following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC33046; MGC33047; Neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; Programmed cell death protein 4; Protein 197/15a; Protein MA-3; RP11-348N5.4; Topoisomerase-inhibitor suppressed protein
Gene Aliases: D19Ucla1; H731; Ma3; PDCD4; Tis
UniProt ID: (Human) Q53EL6, (Mouse) Q61823
Entrez Gene ID: (Human) 27250, (Mouse) 18569
apoptotic process
cell aging
positive regulation of smooth muscle cell apoptotic process
negative regulation of apoptotic process
negative regulation of JUN kinase activity
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of myofibroblast differentiation
regulation of protein metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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