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PDGF-C Antibody (PA5-47141) in IHC (P)
Immunohistochemical analysis of PDGF-C in perfusion fixed paraffin-embedded sections of mouse kidney. Samples were incubated with PDGF-C polyclonal antibody (Product # PA5-47141) using a dilution of 5 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen ... View More
Product Details
PA5-47141
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse PDGF-CC Val235-Gly345
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606896
Product Specific Information
In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) PDGF-CC is observed and less than 1% cross-reactivity with rhPDGF-AA, rhPDGF-BB, rhPDGF-D, and recombinant rat PDGF-AB is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Sphingosine Kinase 2 (Sphk2) catalyzes the phosphorylation of sphingosine to sphingosine 1 phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide range of cellular events including cell motility, survival, apoptosis, migration and cellcell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C76851; Fallotein; MGC102297; PDGF-C; Platelet-derived growth factor C; SCDGF; Spinal cord-derived growth factor; VEGF-E
Gene Aliases: 1110064L01Rik; AI647969; PDGF-C; Pdgfc; Scdgf
UniProt ID: (Mouse) Q8CI19
Entrez Gene ID: (Mouse) 54635
activation of transmembrane receptor protein tyrosine kinase activity
multicellular organism development
positive regulation of cell proliferation
embryo development
organ morphogenesis
positive regulation of DNA replication
platelet-derived growth factor receptor signaling pathway
positive regulation of fibroblast proliferation
digestive tract development
regulation of peptidyl-tyrosine phosphorylation
positive regulation of cell division
bone development
cellular response to amino acid stimulus
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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