Invitrogen

PDGF-D Polyclonal Antibody

PDGF-D Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PDGF-D Antibody (40-2100) in IHC (P)

Immunohistochemistry analysis of PDGF-D showing staining in the cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, wa... View More

PDGF-D Antibody in Immunohistochemistry (IHC)

PDGF-D Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Product Details

40-2100

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of the human PDGF-D

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533455

Target Information

The platelet-derived growth factor (PDGF) family consists of four members; PDGF-A, PDGF-B, PDGF-C and PDGF-D (spinal cord-derived growth factor-B or iris-expressed growth factor). PDGF members form disulphide-bonded dimeric isoforms, which are important for growth, survival and function in several types of connective tissue cells. Their biological effects are mediated via two tyrosine kinase receptors, PDGFR-a and PDGFR-b, and PDGF-mediated signaling is critical for development of many organ systems. PDGF-D has a two-domain structure similar to PDGF-C and is secreted as a disulphide-linked homodimer, PDGF-DD. PDGF-D induces cellular transformation and promotes tumor growth by accelerating the proliferation rate of tumor cells and by stimulation of tumor neovascularization. PDGF-D may play a role in the development of brain tumors. The potential oncogenic activity of PDGF-DD may be important for the development and/or progression of prostate cancer. PDGF-D is expressed in fibroblastic adventitial cells, cultured endothelial cells and in a variety of tumor cell lines including those derived from ovarian, renal and lung cancers, as well as from astrocytomas and medulloblastomas. PDGF-D is expressed in the human kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SU16f inhibits fibrotic scar formation and facilitates axon regeneration and locomotor function recovery after spinal cord injury by blocking the PDGFRβ pathway.

Journal of neuroinflammation

Li Z,Yu S,Liu Y,Hu X,Li Y,Xiao Z,Chen Y,Tian D,Xu X,Cheng L,Zheng M,Jing J

Published figure using PDGF-D polyclonal antibody (Product # 40-2100) in Immunohistochemistry

Sat Apr 16 00:00:00 UTC 2022

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.

The Journal of experimental medicine

Cheng YW,Zhang ZB,Lan BD,Lin JR,Chen XH,Kong LR,Xu L,Ruan CC,Gao PJ

40-2100 was used in Immunohistochemistry (Paraffin), Western Blot to provide for the first time a new interaction pattern between macrophage and adipocyte: that macrophage-derived uPA activates adipocyte-secreted PDGF-D, which finally accelerates AngII-induced cardiac remodeling in obese mice.

Mon Sep 06 00:00:00 UTC 2021

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.

The Journal of experimental medicine

Cheng YW,Zhang ZB,Lan BD,Lin JR,Chen XH,Kong LR,Xu L,Ruan CC,Gao PJ

Mon Sep 06 00:00:00 UTC 2021

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.

The Journal of experimental medicine

Cheng YW,Zhang ZB,Lan BD,Lin JR,Chen XH,Kong LR,Xu L,Ruan CC,Gao PJ

Mon Sep 06 00:00:00 UTC 2021

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice.

The Journal of experimental medicine

Cheng YW,Zhang ZB,Lan BD,Lin JR,Chen XH,Kong LR,Xu L,Ruan CC,Gao PJ

Mon Sep 06 00:00:00 UTC 2021

Cytomegalovirus promotes murine glioblastoma growth via pericyte recruitment and angiogenesis.

The Journal of clinical investigation

Krenzlin H,Behera P,Lorenz V,Passaro C,Zdioruk M,Nowicki MO,Grauwet K,Zhang H,Skubal M,Ito H,Zane R,Gutknecht M,Griessl MB,Ricklefs F,Ding L,Peled S,Rooj A,James CD,Cobbs CS,Cook CH,Chiocca EA,Lawler SE

Published figure using PDGF-D polyclonal antibody (Product # 40-2100) in Immunohistochemistry

Mon Mar 11 00:00:00 UTC 2019

Cytomegalovirus promotes murine glioblastoma growth via pericyte recruitment and angiogenesis.

The Journal of clinical investigation

Published figure using PDGF-D polyclonal antibody (Product # 40-2100) in Immunohistochemistry

Mon Mar 11 00:00:00 UTC 2019

Overexpression of matriptase in tumor stroma is a poor prognostic indicator of extrahepatic bile duct cancer.

Pathology international

Komatsubara T,Oshiro H,Sakuma Y,Sata N,Niki T,Fukushima N

40-2100 was used in Immunohistochemistry to evaluate the clinicopathological significance of platelet derived growth factor D in bile duct cancer.

Fri Feb 01 00:00:00 UTC 2019

Hyperhomocysteinemia Causes Chorioretinal Angiogenesis with Placental Growth Factor Upregulation.

Scientific reports

Lee YJ,Ke CY,Tien N,Lin PK

40-2100 was used in Western Blotting to examine homocysteine-related choroidal angiogenesis and possible related factors.

Thu Oct 25 00:00:00 UTC 2018

Hyperhomocysteinemia Causes Chorioretinal Angiogenesis with Placental Growth Factor Upregulation.

Scientific reports

Lee YJ,Ke CY,Tien N,Lin PK

40-2100 was used in Western Blotting to examine homocysteine-related choroidal angiogenesis and possible related factors.

Thu Oct 25 00:00:00 UTC 2018

Molecular signature of epithelial-mesenchymal transition (EMT) in human prostate cancer bone metastasis.

American journal of translational research

Sethi S,Macoska J,Chen W,Sarkar FH

40-2100 was used in Immunohistochemistry to suggest that Notch-1 could play a role in prostate cancer bone metastasis.

Sat Oct 23 00:00:00 UTC 2010

Platelet-derived growth factor isoform expression in carbon tetrachloride-induced chronic liver injury.

Laboratory investigation; a journal of technical methods and pathology

Borkham-Kamphorst E,Kovalenko E,van Roeyen CR,Gassler N,Bomble M,Ostendorf T,Floege J,Gressner AM,Weiskirchen R

40-2100 was used in immunohistochemistry - paraffin section to measure the expression of PDGF isoforms and receptors in chronic liver injury in vivo

Wed Oct 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: Iris-expressed growth factor; PDGF-D; Platelet-derived growth factor D; platelet-derived growth factor, D polypeptide; SCDGF-B; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B; spinal-cord derived growth factor-B protein

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Gene Aliases: 1110003I09Rik; IEGF; MSTP036; PDGFD; rSCDGF-B; SCDGF-B; SCDGFB; UNQ1899/PRO4345

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UniProt ID: (Human) Q9GZP0, (Rat) Q9EQT1, (Mouse) Q925I7

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Entrez Gene ID: (Human) 80310, (Rat) 66018, (Mouse) 71785

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Function(s)

platelet-derived growth factor receptor binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PDGF-D Polyclonal Antibody

PDGF-D Antibody (40-2100) in IHC (P)

Product Details

40-2100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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