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Invitrogen
PDHA2 Polyclonal Antibody
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FIGURE: 1 / 4
PDHA2 Antibody (PA5-106454) in ICC/IF
Immunocytochemistry analysis of PDHA2 in HepG2 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PDHA2 polyclonal antibody (Product # PA5-106454) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106454
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PDHA2(Accession P29803), corresponding to amino acid residues L333-K383.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854125
Product Specific Information
Antibody detects endogenous levels of total PDHA2.
Target Information
PDHA2 (Pyruvate Dehydrogenase E1 Alpha 2 Subunit) is a Protein Coding gene. Diseases associated with PDHA2 include Spermatogenic Failure 1. Among its related pathways are Citrate cycle (TCA cycle)and Central carbon metabolism in cancer. Gene Ontology (GO) annotations related to this gene include oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptorandpyruvate dehydrogenase (acetyl-transferring) activity. An important paralog of this gene isPDHA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PDHE1-A type II; pyruvate dehydrogenase; pyruvate dehydrogenase E1 alpha 2; PYRUVATE DEHYDROGENASE E1 COMPONENT ALPHA SUBUNIT, TESTIS-SPECIFIC FORM, MITOCHONDRIAL PRECURSOR (PDHE1-A TYPE II); Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; pyruvate dehydrogenase, E1-alpha polypeptide, testis specific
Gene Aliases: Pdha-2; PDHA2; PDHAL
UniProt ID: (Human) P29803, (Mouse) P35487, (Rat) Q06437
Entrez Gene ID: (Human) 5161, (Mouse) 18598, (Rat) 117098
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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