Proteintech

PDI Monoclonal Antibody (2E6A11)

PDI Antibody in Immunocytochemistry (ICC/IF)

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PDI Antibody (66422-1-IG) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (Product # 66422-1-IG, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

66422-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10,000-1:100,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:50-1:500

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2E6A11

Immunogen

PDI Fusion Protein Ag1747 (89-273 aa encoded by BC014504)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTKYKPESE ELTAEWITEF CHRFLEGKIK PHLMSQELPE DWDKQPVKVL VGKNFEDVAF DEKKNVFVEF YAPWCGHCKQ LAPIWDKLGE TYKDHENIVI AKMDSTANEV EAVKVHSFPT LKFFPASADR TVIDYNGERT LDGFKKFLES GGQDGAGDDD DLEDLEEAEE PDMEEDDDQK AVKDEL (89-273 aa encoded by BC014504)

Target Information

Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Periostin Protects Against Alcohol-related Liver Disease by Activating Autophagy by Interacting With Protein Disulfide Isomerase.

Cellular and molecular gastroenterology and hepatology

Zhang Y,Jin J,Wu H,Huang J,Ye S,Qiu J,Ouyang G,Wu T,Liu F,Liu Y

Mon May 22 00:00:00 UTC 2023

Periostin Protects Against Alcohol-related Liver Disease by Activating Autophagy by Interacting With Protein Disulfide Isomerase.

Cellular and molecular gastroenterology and hepatology

Zhang Y,Jin J,Wu H,Huang J,Ye S,Qiu J,Ouyang G,Wu T,Liu F,Liu Y

Mon May 22 00:00:00 UTC 2023

Dickkopf-1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li X,Zheng T,Zhang Y,Zhao Y,Liu F,Dai S,Liu X,Zhang M

Tue Aug 01 00:00:00 UTC 2023

Identification and characterization of a new variation in DPM2 gene in two Chinese siblings with mild intellectual impairment.

Frontiers in genetics

Zhao P,Hu Y,Hu J,Li C,Huang Y,Zhang L,Luo S,Zhu H,Jiang J,He X

Tue May 09 00:00:00 UTC 2023

SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization.

Biochimica et biophysica acta. Molecular cell research

Wiertelak W,Chabowska K,Szulc B,Zadorozhna Y,Olczak M,Maszczak-Seneczko D

Thu Jun 01 00:00:00 UTC 2023

Sigma‑1 receptor overexpression promotes proliferation and ameliorates cell apoptosis in β‑cells.

Molecular medicine reports

Ke M,Lin F,Wang H,He G,Feng J,Song L,Xu Y,Liu J

Sun May 01 00:00:00 UTC 2022

Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels.

Biochimica et biophysica acta. General subjects

Huang T,Wu Q,Huang H,Zhang C,Wang L,Wang L,Liu Y,Li W,Zhang J,Liu Y

Sat Jan 01 00:00:00 UTC 2022

CIDEA Regulates De Novo Fatty Acid Synthesis in Bovine Mammary Epithelial Cells by Targeting the AMPK/PPARγ Axis and Regulating SREBP1.

Journal of agricultural and food chemistry

Cheng J,Xu D,Chen L,Guo W,Hu G,Liu J,Fu S

Wed Sep 14 00:00:00 UTC 2022

A Splice Switch in SIGIRR Causes a Defect of IL-37-Dependent Anti-Inflammatory Activity in Cystic Fibrosis Airway Epithelial Cells.

International journal of molecular sciences

Ueno-Shuto K,Kamei S,Hayashi M,Fukuyama A,Uchida Y,Tokutomi N,Suico MA,Kai H,Shuto T

Wed Jul 13 00:00:00 UTC 2022

A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function.

Journal of cell science

McCaughey J,Stevenson NL,Mantell JM,Neal CR,Paterson A,Heesom K,Stephens DJ

Wed Sep 01 00:00:00 UTC 2021

Highly Efficient Method for Intracellular Delivery of Proteins Mediated by Cholera Toxin-Induced Protein Internalization.

Molecular pharmaceutics

Jia X,Zhang Y,Wang T,Fu Y

Mon Nov 01 00:00:00 UTC 2021

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Cell death & disease

Wang P,Luo R,Zhang M,Wang Y,Song T,Tao T,Li Z,Jin L,Zheng H,Chen W,Zhao M,Zheng Y,Qin J

Tue Dec 08 00:00:00 UTC 2020

HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression.

Cellular signalling

Liu L,Long H,Wu Y,Li H,Dong L,Zhong JL,Liu Z,Yang X,Dai X,Shi L,Ren M,Lin Z

Mon Oct 01 00:00:00 UTC 2018

HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression.

Cellular signalling

Liu L,Long H,Wu Y,Li H,Dong L,Zhong JL,Liu Z,Yang X,Dai X,Shi L,Ren M,Lin Z

Mon Oct 01 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: CaO19.12595; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; glutathione-insulin transhydrogenase; p55; PDI; PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase family A, member 1; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55

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Gene Aliases: CLCRP1; DSI; ERBA2L; ERp59; GIT; P4HB; P4Hbeta; PDI; PDIA1; PDIR; PHDB; PO4DB; PO4HB; PROHB; Thbp

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UniProt ID: (Human) P07237, (Rat) P04785, (Mouse) P09103

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Entrez Gene ID: (Human) 5034, (Rat) 25506, (Mouse) 18453

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Function(s)

protein disulfide isomerase activity procollagen-proline 4-dioxygenase activity integrin binding protein binding enzyme binding poly(A) RNA binding protein heterodimerization activity peptidyl-proline 4-dioxygenase activity isomerase activity

Process(es)

protein folding peptidyl-proline hydroxylation to 4-hydroxy-L-proline response to endoplasmic reticulum stress cell redox homeostasis cellular response to hypoxia regulation of oxidative stress-induced intrinsic apoptotic signaling pathway response to reactive oxygen species lipoprotein biosynthetic process positive regulation of viral entry into host cell

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PDI Monoclonal Antibody (2E6A11)

PDI Antibody (66422-1-IG) in ICC/IF

Product Details

66422-1-IG

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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