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          • Primary Antibodies ›
          • PDI Antibodies

          Invitrogen

          PDI Monoclonal Antibody (RL90)

          Advanced Verification
          58 Published Figures
          88 References
          View all (34) PDI antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite PDI Monoclonal Antibody (RL90)

          • Antibody Testing Data (18)
          • Published Figures (58)
          • Advanced Verification (2)
          PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 78

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          PDI Antibody (MA3-019) in IHC (P)

          Immunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Flow Cytometry (Flow)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Flow Cytometry (Flow)
          PDI Antibody in Flow Cytometry (Flow)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunocytochemistry (ICC/IF)
          PDI Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDI Antibody in Immunohistochemistry (IHC)
          PDI Antibody in Immunohistochemistry (IHC)
          PDI Antibody in Immunohistochemistry (IHC)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Western Blot (WB)
          PDI Antibody in Neutralization (Neu)
          PDI Antibody in Neutralization (Neu)
          PDI Antibody
          PDI Antibody

          Product Details

          MA3-019

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200-1:2,000
          View 22 publications 22 publications

          Immunohistochemistry (IHC)

          -
          View 12 publications 12 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          1:100
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          View 42 publications 42 publications

          Flow Cytometry (Flow)

          0.5-1 µg/test
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          Assay-dependent
          View 3 publications 3 publications

          Neutralization (Neu)

          -
          View 5 publications 5 publications

          Immunomicroscopy (IM)

          Assay-dependent
          -

          Competition Assay (CA)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Hamster, Human, Mouse, Pig, Rat

          Published species

          Dog, Hamster, Human, Mouse, Non-human primate, Rat, Rhesus monkey

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          RL90

          Immunogen

          Purified rat PDI protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 555 
          • Alexa Fluor 647 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2163120

          Product Specific Information

          MA3-019 detects protein disulphide-isomerase (PDI) from human, rat, porcine and mouse tissues as well as hamster cells.

          MA3-019 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a protein at 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-019 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-019 has been shown to inhibit the activity of PDI in vitro. MA3-019 has also been found to inhibit disulfide bond reduction of the HIV protein, gp120, at the cell surface of CHO cells and human lymphoid cells.

          In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.

          The MA3-019 antigen is purified rat PDI.

          Target Information

          Protein disulfide isomerase (PDI) is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, the protein seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. It may therefore cause structural modifications of exofacial proteins. Inside the cell, PDI seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, it functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). PDI may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CaO19.12595; Cellular thyroid hormone-binding protein; collagen prolyl 4-hydroxylase beta; Endoplasmic reticulum resident protein 59; Eps1p; ER protein 59; glutathione-insulin transhydrogenase; p55; p58; PDI; PDI1P; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; Prolyl 4-hydroxylase subunit beta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide isomerase; Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide); protein disulfide isomerase family A, member 1; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; Protein disulfide-isomerase; protocollagen hydroxylase; testicular secretory protein Li 32; thyroid hormone-binding protein p55

          View more View less

          Gene Aliases: CLCRP1; DSI; ERBA2L; ERp59; GIT; I79_002399; P4HB; P4Hbeta; p58; PDI; PDIA1; PDIR; PHDB; PO4DB; PO4HB; PROHB; Thbp

          View more View less

          UniProt ID: (Human) P07237, (Rat) P04785, (Mouse) P09103, (Chinese hamster) Q8R4U2

          View more View less

          Entrez Gene ID: (Human) 5034, (Rat) 25506, (Mouse) 18453, (Chinese hamster) 100689433

          View more View less

          Function(s)
          protein disulfide isomerase activity procollagen-proline 4-dioxygenase activity integrin binding protein binding enzyme binding poly(A) RNA binding protein heterodimerization activity peptidyl-proline 4-dioxygenase activity isomerase activity
          Process(es)
          protein folding peptidyl-proline hydroxylation to 4-hydroxy-L-proline response to endoplasmic reticulum stress cell redox homeostasis cellular response to hypoxia regulation of oxidative stress-induced intrinsic apoptotic signaling pathway response to reactive oxygen species lipoprotein biosynthetic process positive regulation of viral entry into host cell
          It has to be done as per old AB suggested Products section.
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