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Invitrogen
PDIR Polyclonal Antibody
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FIGURE: 1 / 10
PDIR Antibody (PA5-144006) in ICC/IF
Immunocytochemistry analysis of PDIR in Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PDIR Polyclonal antibody (Product # PA5-144006) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
PA5-144006
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PDIA5 recombinant protein (Position: R46-Y481).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_3075220
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell, human THP-1 whole cell, human MCF-7 whole cell, human HL-60 whole cell, human A431 whole cell, human T47D whole cell, rat liver tissue, rat lung tissue, rat stomach tissue, rat pancreas tissue, mouse liver tissue, mouse lung tissue, mouse stomach tissue, mouse pancreas tissue. IHC: human breast cancer tissue, human placenta tissue, human gallbladder adenocarcinoma tissue, human rectal cancer tissue, human lung cancer tissue, human ovarian adenocarcinoma tissue, human adrenocortical adenoma tissue. ICC/IF: CACO-2 cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
PDIR (protein disulfide isomerase-related protein) is a novel PDI protein that was originally isolated from a human placental cDNA library. It has been suggested that from its nucleotide sequence, PDIR has three CXXC-like motifs (Cys-Ser-Met-Cys, Cys-Gly-His-Cys and Cys-Pro-His-Cys), which are found in the PDI superfamily of proteins. The CXXC motif is responsible for oxidoreductase activity. PDIR is also shown to have a putative endoplasmic reticulum (ER) retention signal 'Lys-Glu-Glu-Leu' at its carboxyl terminus, indicative of ER resident proteins. Northern blot analysis of PDIR indicates that it is preferentially expressed in cells actively secreting proteins. It has also been shown that expression of PDIR is stress-inducible. Data suggest that PDIR, like other PDIs, has oxidoreductase activity of disulfide bonds and facilitates the protein folding process within the lumen of the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: protein disulfide isomerase A5; protein disulfide isomerase family A, member 5; protein disulfide isomerase-associated 5; Protein disulfide isomerase-related protein; Protein disulfide-isomerase A5
Gene Aliases: 2700053F16Rik; AU015525; PDIA5; PDIR
UniProt ID: (Human) Q14554, (Mouse) Q921X9, (Rat) Q5I0H9
Entrez Gene ID: (Human) 10954, (Mouse) 72599, (Rat) 360722
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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