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PDIR Antibody (PA5-89796) in WB
Western blot analysis of PDIR in extracts of various cell lines. Samples were incubated with PDIR Polyclonal antibody (Product # PA5-89796) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-89796
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-140 of human PDIA5 (NP_0068011)
Conjugate
Form
Liquid
Concentration
1.78 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805780
Product Specific Information
Immunogen sequence: SSAKVSSLIE RISDPKDLKK LLRTRNNVLV LYSKSEVAAE NHLRLLSTVA QAVKGQGTIC WVDCGDAESR KLCKKMKVDL SPKDKKVELF HYQDGAFHTE YNRAVTFKSI VAFLKDPKG; Positive Samples: Mouse kidney, Mouse liver, Mouse pancreas, Rat liver; Cellular Location: Endoplasmic reticulum lumen
Target Information
PDIR (protein disulfide isomerase-related protein) is a novel PDI protein that was originally isolated from a human placental cDNA library. It has been suggested that from its nucleotide sequence, PDIR has three CXXC-like motifs (Cys-Ser-Met-Cys, Cys-Gly-His-Cys and Cys-Pro-His-Cys), which are found in the PDI superfamily of proteins. The CXXC motif is responsible for oxidoreductase activity. PDIR is also shown to have a putative endoplasmic reticulum (ER) retention signal 'Lys-Glu-Glu-Leu' at its carboxyl terminus, indicative of ER resident proteins. Northern blot analysis of PDIR indicates that it is preferentially expressed in cells actively secreting proteins. It has also been shown that expression of PDIR is stress-inducible. Data suggest that PDIR, like other PDIs, has oxidoreductase activity of disulfide bonds and facilitates the protein folding process within the lumen of the ER.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: protein disulfide isomerase A5; protein disulfide isomerase-associated 5; Protein disulfide isomerase-related protein; Protein disulfide-isomerase A5
Gene Aliases: 2700053F16Rik; AU015525; Pdia5; Pdir
UniProt ID: (Mouse) Q921X9, (Rat) Q5I0H9
Entrez Gene ID: (Mouse) 72599, (Rat) 360722
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