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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PDK4 Antibodies

          Proteintech

          PDK4 Polyclonal Antibody

          47 References
          View all (11) PDK4 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite PDK4 Polyclonal Antibody

          • Antibody Testing Data (15)
          PDK4 Antibody in Immunocytochemistry (ICC/IF)
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          PDK4 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

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          PDK4 Antibody (12949-1-AP) in ICC/IF

          Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 12949-1-AP (PDK4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PDK4 Antibody in Immunocytochemistry (ICC/IF)
          PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDK4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          PDK4 Antibody in Western Blot (WB)
          PDK4 Antibody in Western Blot (WB)
          PDK4 Antibody in Western Blot (WB)
          PDK4 Antibody in Western Blot (WB)
          PDK4 Antibody in Western Blot (WB)
          PDK4 Antibody in Western Blot (WB)
          PDK4 Antibody in Western Blot (WB)
          PDK4 Antibody in Western Blot (WB)

          Product Details

          12949-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:3,000
          View 39 publications 39 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          PDK4 Fusion Protein Ag3629 (61-412 aa encoded by BC040239)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.19 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: LPVRLANIL KEIDILPTQL VNTSSVQLVK SWYIQSLMDL VEFHEKSPDD QKALSDFVDT LIKVRNRHHN VVPTMAQGII EYKDACTVDP VTNQNLQYFL DRFYMNRIST RMLMNQHILI FSDSQTGNPS HIGSIDPNCD VVAVVQDAFE CSRMLCDQYY LSSPELKLTQ VNGKFPDQPI HIVYVPSHLH HMLFELFKNA MRATVEHQEN QPSLTPIEVI VVLGKEDLTI KISDRGGGVP LRIIDRLFSY TYSTAPTPVM DNSRNAPLAG FGYGLPISRL YAKYFQGDLN LYSLSGYGTD AIIYLKALSS ESIEKLPVFN KSAFKHYQMS SEADDWCIPS REPKNLAKEV AM (61-412 aa encoded by BC040239)

          Target Information

          PDK4 (PDHK4), along with the other PDHKs, regulates glucose metabolism by phosphorylating the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex. Inhibition of PDHKs is viewed as a potential therapeutic treatment for type II diabetes. This protein is located in the matrix of the mitrochondria and it's expression is regulated by glucocorticoids, retinoic acid and insulin. PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; kinase isozyme 4; lipoamide; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 4; Pyruvate dehydrogenase kinase isoform 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase kinase, isozyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; pyruvate dehydrogenate kinase 4

          View more View less

          Gene Aliases: AV005916; PDHK4; PDK4

          View more View less

          UniProt ID: (Human) Q16654, (Rat) O54937, (Mouse) O70571

          View more View less

          Entrez Gene ID: (Human) 5166, (Rat) 89813, (Mouse) 27273

          View more View less

          Function(s)
          protein kinase activity pyruvate dehydrogenase (acetyl-transferring) kinase activity ATP binding nucleotide binding protein serine/threonine/tyrosine kinase activity kinase activity transferase activity
          Process(es)
          positive regulation of defense response to virus by host glucose metabolic process protein phosphorylation regulation of pH insulin receptor signaling pathway cellular response to starvation regulation of acetyl-CoA biosynthetic process from pyruvate regulation of cellular ketone metabolic process regulation of glucose metabolic process regulation of fatty acid biosynthetic process glucose homeostasis response to starvation regulation of bone resorption regulation of fatty acid oxidation cellular response to fatty acid reactive oxygen species metabolic process xenophagy negative regulation of anoikis acetyl-CoA biosynthetic process from pyruvate carbohydrate metabolic process phosphorylation
          It has to be done as per old AB suggested Products section.
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