Proteintech

PDLIM7 Polyclonal Antibody

View all (14) PDLIM7 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (8)

PDLIM7 Antibody in Immunocytochemistry (ICC/IF)

PDLIM7 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

PDLIM7 Antibody (10221-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed SKOV-3 cells using 10221-1-AP (PDLIM7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PDLIM7 Antibody in Immunocytochemistry (ICC/IF)

PDLIM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PDLIM7 Antibody in Western Blot (WB)

PDLIM7 Antibody in Western Blot (WB)

PDLIM7 Antibody in Western Blot (WB)

PDLIM7 Antibody in Western Blot (WB)

PDLIM7 Antibody in Immunoprecipitation (IP)

PDLIM7 Antibody in Immunoprecipitation (IP)

Product Image Gallery

PDLIM7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed SKOV-3 cells using 10221-1-AP (PDLIM7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PDLIM7 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 10221-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 25x lens).

PDLIM7 Antibody in Western Blot (WB) — SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PDLIM7 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PDLIM7 Antibody in Western Blot (WB) — MCF-7 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

PDLIM7 Antibody in Western Blot (WB) — WB result of PDLIM7 antibody (10221-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

PDLIM7 Antibody in Immunoprecipitation (IP) — IP result of anti-PDLIM7,LMP1 (IP:10221-1-AP, 4 ug; Detection:10221-1-AP 1:300) with MCF-7 cells lysate 2000 ug.

PDLIM7 Antibody in Immunoprecipitation (IP) — IP result of PDLIM7 antibody (10221-1-AP for IP and Detection) with HeLa cell lysate.

Product Details

10221-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PDLIM7 Fusion Protein Ag0264 (3-289 aa encoded by BC001093)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SFKVVLEGP APWGFRLQGG KDFNVPLSIS RLTPGGKAAQ AGVAVGDWVL SIDGENAGSL THIEAQNKIR ACGERLSLGL SRAQPVQSKP QKASAPAADP PRYTFAPSVS LNKTARPFGA PPPADSAPQQ NGQPLRPLVP DASKQRLMEN TEDWRPRPGT GQSRSFRILA HLTGTEFMQD PDEEHLKKSS QVPRTEAPAP ASSTPQEPWP GPTAPSPTSR PPWAVDPAFA ERYAPDKTST VLTRHSQPAT PTPLQSRTSI VQAAAGGVPG GGSNNGKTPV CHQCHKVI (3-289 aa encoded by BC001093)

Target Information

The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1.

Cell & bioscience

Wu Z,Ge L,Ma L,Lu M,Song Y,Deng S,Duan P,Du T,Wu Y,Zhang Z,Zhang S

Thu Feb 23 00:00:00 UTC 2023

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1.

Cell & bioscience

Wu Z,Ge L,Ma L,Lu M,Song Y,Deng S,Duan P,Du T,Wu Y,Zhang Z,Zhang S

Thu Feb 23 00:00:00 UTC 2023

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1.

Cell & bioscience

Wu Z,Ge L,Ma L,Lu M,Song Y,Deng S,Duan P,Du T,Wu Y,Zhang Z,Zhang S

Thu Feb 23 00:00:00 UTC 2023

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1.

Cell & bioscience

Wu Z,Ge L,Ma L,Lu M,Song Y,Deng S,Duan P,Du T,Wu Y,Zhang Z,Zhang S

Thu Feb 23 00:00:00 UTC 2023

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1.

Cell & bioscience

Wu Z,Ge L,Ma L,Lu M,Song Y,Deng S,Duan P,Du T,Wu Y,Zhang Z,Zhang S

Thu Feb 23 00:00:00 UTC 2023

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1.

Cell & bioscience

Wu Z,Ge L,Ma L,Lu M,Song Y,Deng S,Duan P,Du T,Wu Y,Zhang Z,Zhang S

Thu Feb 23 00:00:00 UTC 2023

Proteomic identification and structural basis for the interaction between sorting nexin SNX17 and PDLIM family proteins.

Structure (London, England : 1993)

Healy MD,Sacharz J,McNally KE,McConville C,Tillu VA,Hall RJ,Chilton M,Cullen PJ,Mobli M,Ghai R,Stroud DA,Collins BM

Thu Dec 01 00:00:00 UTC 2022

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling.

International journal of medical sciences

Mu R,Chen B,Bi B,Yu H,Liu J,Li J,He M,Rong L,Liu B,Liu K,Zhu L,Shi X,Shuai Y,Jin L

Mon Aug 08 00:00:00 UTC 2022

PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.

Frontiers in immunology

Jodo A,Shibazaki A,Onuma A,Kaisho T,Tanaka T

Tue Apr 20 00:00:00 UTC 2021

Physical and Molecular Landscapes of Mouse Glioma Extracellular Vesicles Define Heterogeneity.

Cell reports

Gyuris A,Navarrete-Perea J,Jo A,Cristea S,Zhou S,Fraser K,Wei Z,Krichevsky AM,Weissleder R,Lee H,Gygi SP,Charest A

Tue Jun 25 00:00:00 UTC 2019

Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification.

EMBO reports

Abend A,Shkedi O,Fertouk M,Caspi LH,Kehat I

Sat Jul 01 00:00:00 UTC 2017

Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification.

EMBO reports

Abend A,Shkedi O,Fertouk M,Caspi LH,Kehat I

Sat Jul 01 00:00:00 UTC 2017

LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2-Mediated Osteogenesis Through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Pan H,Li X,Wang J,Zhang K,Yang H,Li Z,Zheng Z,Liu H

Sat Aug 01 00:00:00 UTC 2015

LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2-Mediated Osteogenesis Through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Pan H,Li X,Wang J,Zhang K,Yang H,Li Z,Zheng Z,Liu H

Sat Aug 01 00:00:00 UTC 2015

LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Liu H,Pan H,Yang H,Wang J,Zhang K,Li X,Wang H,Ding W,Li B,Zheng Z

Sun Mar 01 00:00:00 UTC 2015

LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society

Liu H,Pan H,Yang H,Wang J,Zhang K,Li X,Wang H,Ding W,Li B,Zheng Z

Sun Mar 01 00:00:00 UTC 2015

Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation.

Cytotechnology

Zhu Z,Liu Z,Liu J,Bi M,Yang T,Wang J

Sun Mar 01 00:00:00 UTC 2015

Proteomic profiling of human placenta-derived mesenchymal stem cells upon transforming LIM mineralization protein-1 stimulation.

Cytotechnology

Zhu Z,Liu Z,Liu J,Bi M,Yang T,Wang J

Sun Mar 01 00:00:00 UTC 2015

LIM mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells.

International journal of molecular sciences

Liu H,Huang L,Zhang Z,Zhang Z,Yu Z,Chen X,Chen Z,Zen Y,Yang D,Han Z,Shu Y,Dai M,Cao K

Wed Apr 23 00:00:00 UTC 2014

LIM mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells.

International journal of molecular sciences

Liu H,Huang L,Zhang Z,Zhang Z,Yu Z,Chen X,Chen Z,Zen Y,Yang D,Han Z,Shu Y,Dai M,Cao K

Wed Apr 23 00:00:00 UTC 2014

LIM mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells.

International journal of molecular sciences

Liu H,Huang L,Zhang Z,Zhang Z,Yu Z,Chen X,Chen Z,Zen Y,Yang D,Han Z,Shu Y,Dai M,Cao K

Wed Apr 23 00:00:00 UTC 2014

LIM mineralization protein-1 inhibits the malignant phenotypes of human osteosarcoma cells.

International journal of molecular sciences

Liu H,Huang L,Zhang Z,Zhang Z,Yu Z,Chen X,Chen Z,Zen Y,Yang D,Han Z,Shu Y,Dai M,Cao K

Wed Apr 23 00:00:00 UTC 2014

Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development.

Journal of molecular histology

Fang P,Wang X,Zhang L,Yuan G,Chen Z,Zhang Q

Fri Oct 01 00:00:00 UTC 2010

Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development.

Journal of molecular histology

Fang P,Wang X,Zhang L,Yuan G,Chen Z,Zhang Q

Fri Oct 01 00:00:00 UTC 2010

Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.

Journal of endodontics

Wang X,Zhang Q,Chen Z,Zhang L

Fri Feb 01 00:00:00 UTC 2008

Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.

Journal of endodontics

Wang X,Zhang Q,Chen Z,Zhang L

Fri Feb 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: 1110003B01Rik; LIM domain protein; LIM mineralization protein; Lim mineralization protein 3; LMP; PDZ and LIM domain 7 (enigma); PDZ and LIM domain protein 7; Protein enigma

View more View less

Gene Aliases: ENIGMA; LMP1; LMP3; PDLIM7

View more View less

UniProt ID: (Human) Q9NR12

View more View less

Entrez Gene ID: (Human) 9260

View more View less

protein binding zinc ion binding

ossification receptor-mediated endocytosis multicellular organism development actin cytoskeleton organization cell differentiation positive regulation of osteoblast differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support