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Invitrogen
PDPK1 Recombinant Rabbit Monoclonal Antibody (SD204-9)
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Product Details
MA5-32354
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD204-9
Immunogen
Synthetic peptide within Human PDPK1 aa 1-50
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2809635
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PkB kinase; PkB kinase like gene 1; PkB-like 1; Protein kinase B kinase
Gene Aliases: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461
UniProt ID: (Human) O15530, (Mouse) Q9Z2A0, (Rat) O55173
Entrez Gene ID: (Human) 5170, (Mouse) 18607, (Rat) 81745
protein serine/threonine kinase activity
3-phosphoinositide-dependent protein kinase activity
insulin receptor binding
protein binding
ATP binding
phospholipase activator activity
kinase activity
protein kinase binding
phospholipase binding
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
type B pancreatic cell development
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
negative regulation of protein kinase activity
hyperosmotic response
signal transduction
epidermal growth factor receptor signaling pathway
positive regulation of phospholipase activity
regulation of endothelial cell migration
negative regulation of cardiac muscle cell apoptotic process
phosphorylation
cell migration
peptidyl-threonine phosphorylation
calcium-mediated signaling
negative regulation of transforming growth factor beta receptor signaling pathway
activation of protein kinase B activity
cellular response to insulin stimulus
negative regulation of toll-like receptor signaling pathway
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of mast cell degranulation
focal adhesion assembly
positive regulation of release of sequestered calcium ion into cytosol
cellular response to epidermal growth factor stimulus
positive regulation of establishment of protein localization to plasma membrane
extrinsic apoptotic signaling pathway
stimulatory C-type lectin receptor signaling pathway
peptidyl-serine phosphorylation
actin cytoskeleton organization
platelet activation
T cell costimulation
intracellular signal transduction
Fc-epsilon receptor signaling pathway
protein autophosphorylation
phosphatidylinositol-mediated signaling
T cell receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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