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FIGURE: 1 / 1
PEDF Antibody (PA5-88254) in WB
Western blot analysis of PEDF in extracts of various cell lines. Samples were incubated with PEDF Polyclonal antibody (Product # PA5-88254) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88254
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 141-240 of human PEDF/SERPINF1 (NP_0026063)
Conjugate
Form
Liquid
Concentration
1.27 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804779
Product Specific Information
Positive Samples: Mouse liver, Mouse kidney, Rat liver; Cellular Location: Melanosome
Immunogen sequence: RIVFEKKLRI KSSFVAPLEK SYGTRPRVLT GNPRLDLQEI NNWVQAQMKG KLARSTKEIP DEISILLLGV AHFKGQWVTK FDSRKTSLED FYLDEERTVR
Target Information
PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors, such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity (1) should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of vasculature-related neurodegenerative diseases, such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-2 antiplasmin; Caspin; Cell proliferation-inducing gene 35 protein; EPC 1; EPC-1; EPC1; PEDF; PIG 35; pigment epithelium derived factor; Pigment epithelium-derived factor; SDF-3; serine (or cysteine) peptidase inhibitor, clade F, member 1; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serine (or cysteine) proteinase inhibitor, clade F), member 1; serine (or cysteine) proteinase inhibitor, clade F, member 1; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serpin-F1; SERPINF 1; sserpin family F member 1; stromal cell derived factor 3; Stromal cell-derived factor 3; testis tissue sperm-binding protein Li 70n
Gene Aliases: AI195227; Dmrs91; EPC-1; OI12; OI6; PEDF; Pedfl; PIG35; Sdf3; SERPINF1
UniProt ID: (Human) P36955, (Mouse) P97298
Entrez Gene ID: (Human) 5176, (Mouse) 20317, (Rat) 287526
short-term memory
negative regulation of endothelial cell migration
negative regulation of gene expression
negative regulation of endopeptidase activity
positive regulation of neuron projection development
negative regulation of angiogenesis
negative regulation of inflammatory response
positive regulation of neurogenesis
negative regulation of epithelial cell proliferation involved in prostate gland development
negative regulation of neuron death
kidney development
aging
response to acidic pH
ovulation cycle
response to arsenic-containing substance
retina development in camera-type eye
cellular response to cobalt ion
cellular response to retinoic acid
cellular response to glucose stimulus
cellular response to dexamethasone stimulus
multicellular organism development
cell proliferation
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