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PEF1 Antibody (PA5-114395) in IHC (P)
Immunohistochemistry analysis of PEF1 in paraffin-embedded human colon cancer tissues. Antigen retrieval was performed with citrate buffer (pH6, 20 min). Samples were blocked with 10% goat serum and incubated in PEF1 polyclonal antibody (Product # PA5-114395) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-rabbit IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB... View More
Product Details
PA5-114395
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PEF1 recombinant protein (Position: R170-L284).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884851
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HEK293 whole cell, human placenta tissue, human PC-3 whole cell, human U-87MG whole cell, human U-937 whole cell, human HepG2 whole cell, human A431 whole cell, human U2OS whole cell, rat lung tissues, rat testicular tissue, mouse lung tissue, mouse testicular tissue. IHC: mouse testis tissue, rat testis tissue, human colon cancer tissue, human lung cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1), sorcin (SRI), grancalcin (GCA), and ALG2. PEF1 is a Ca(2+)-binding protein that belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit (CAPNS1), sorcin (SRI), grancalcin (GCA), and ALG2 (PDCD6) (Kitaura et al., 2001 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PEF protein with a long N-terminal hydrophobic domain; Peflin; penta-EF hand domain containing 1; Penta-EF hand domain-containing protein 1
Gene Aliases: 2600002E23Rik; ABP32; PEF1; PEF1A; Peflin; UNQ1845/PRO3573
UniProt ID: (Human) Q9UBV8, (Mouse) Q8BFY6, (Rat) Q641Z8
Entrez Gene ID: (Human) 553115, (Mouse) 67898, (Rat) 297900
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