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PF1 Antibody (A301-647A) in WB
Detection of human and mouse PF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PF1 antibody (Product # A301-647A) (lot A301-647A-2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A301-647A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 954 and 1004 of human PHD factor 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional TLE5 complexes to DNA. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Pf1; PHD factor 1; PHD finger protein 12; PHD zinc finger transcription factor; PHF12; PHF12 (HGNC:20816)
Gene Aliases: 2410142K10Rik; F630045O13; KIAA1523; mKIAA1523; PF1; PHF12
UniProt ID: (Human) Q96QT6, (Mouse) Q5SPL2
Entrez Gene ID: (Human) 57649, (Mouse) 268448
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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