Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
PF4 Antibody (500-P05-1MG) in WB
Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis PF4 Polyclonal Antibody (Product # 500-P05-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P05-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human PF-4 (CXCL4)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929160
Product Specific Information
AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography employing an immobilized Human PF-4 matrix.
Sandwich ELISA: To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 µL/well) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human PF-4 (CXCL4).
Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; Iroplact; Oncostatin-A; PF-4; Platelet factor 4
Gene Aliases: CXCL4; PF-4; PF4; SCYB4
UniProt ID: (Human) P02776
Entrez Gene ID: (Human) 5196
platelet degranulation
positive regulation of leukocyte chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
negative regulation of angiogenesis
cytokine-mediated signaling pathway
platelet activation
leukocyte chemotaxis
positive regulation of cAMP metabolic process
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
negative regulation of MHC class II biosynthetic process
positive regulation of macrophage differentiation
negative regulation of megakaryocyte differentiation
negative regulation of cytolysis
positive regulation of transcription from RNA polymerase II promoter
chemokine-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support