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PF4 Antibody (MA5-23868) in WB
Western blot analysis of PF4 in mouse platelets. Samples were incubated in PF4 monoclonal antibody (Product # MA5-23868) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for CXCL4/PF4 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
MA5-23868
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
140910
Immunogen
E. coli-derived recombinant mouse CXCL4/PF4 Val30-Ser105
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576249
Product Specific Information
In ELISAs, this antibody does not cross-react or interfere recombinant human (rh) CXCL/PF4, recombinant mouse (rm) CXCL9/MIG, rmGM-CSF, rmCXCL11/I-TAC, rhCXCL8/IL-8, rmIL-5, or rhCXCL10/IP-10. In Western blots, no cross-reactivity was observed with rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rhMIP-1 gamma, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, or rmMIP-3 beta,
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; PF-4; Platelet factor 4
Gene Aliases: Cxcl4; Pf4; Scyb4
UniProt ID: (Mouse) Q9Z126
Entrez Gene ID: (Mouse) 56744
positive regulation of leukocyte chemotaxis
protein complex assembly
chemotaxis
inflammatory response
immune response
G-protein coupled receptor signaling pathway
positive regulation of gene expression
positive regulation of macrophage derived foam cell differentiation
negative regulation of angiogenesis
cytokine-mediated signaling pathway
platelet activation
leukocyte chemotaxis
positive regulation of cAMP metabolic process
response to lipopolysaccharide
positive regulation of tumor necrosis factor production
regulation of cell proliferation
positive regulation of cAMP-mediated signaling
negative regulation of MHC class II biosynthetic process
positive regulation of macrophage differentiation
negative regulation of megakaryocyte differentiation
negative regulation of cytolysis
positive regulation of transcription from RNA polymerase II promoter
cell chemotaxis
chemokine-mediated signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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