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Invitrogen
PFKL Polyclonal Antibody
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Product Details
PA5-21685
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 428 and 674 of PFKL (Uniprot ID#P17858)
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11155219
Product Specific Information
Recommended positive controls: A431, H1299, HeLa, HepG2.
Predicted reactivity: Mouse (95%), Rat (95%), Chicken (88%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type; ATP-PFK; EC 2.7.1.11; K6PL; liver-type 1-phosphofructokinase; PFK-B; Phosphofructo-1-kinase isozyme B; phosphofructokinase 1; Phosphohexokinase
Gene Aliases: AA407869; ATP-PFK; PFK-B; PFK-L; Pfkb; PFKL
UniProt ID: (Human) P17858, (Mouse) P12382
Entrez Gene ID: (Human) 5211, (Mouse) 18641
fructose 6-phosphate metabolic process
glycolytic process
response to glucose
fructose 1,6-bisphosphate metabolic process
negative regulation of insulin secretion
protein oligomerization
protein homotetramerization
canonical glycolysis
carbohydrate metabolic process
metabolic process
phosphorylation
carbohydrate phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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