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FIGURE: 1 / 1
PGCF Antibody (PA5-89697) in WB
Western blot analysis of PGCF in extracts of mouse eye. Samples were incubated with PGCF Polyclonal antibody (Product # PA5-89697) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89697
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-460 of human GUCY2F (NP_0015132)
Conjugate
Form
Liquid
Concentration
1.59 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805731
Product Specific Information
Immunogen sequence: HSALIGGETQ MHLLECAHDL KMTDGTYVFV PYDALLYSLP YKHTPYRVLR NNPKLREAYD AVLTITVESQ EKTFYQAFTE AAARGEIPEK LEFDQVSPLF GTIYNSIYFI AQAMNNAMKE NGQAGAASLV QHSRNMQFHG FNQLMRTDSN GNGISEYVIL DTNLKEWELH STYTVDMEME LLRFGGTPIH FPGGRPPRAD AKCWFAEGKI C; Positive Samples: Mouse eye; Cellular Location: Membrane, Single-pass type I membrane protein
Target Information
GUCY2F (Guanylate cyclase 2F, retinal) is also named as GUC2F, RETGC2 and belongs to the adenylyl cyclase class-4/guanylyl cyclase family. GUCY2F may play a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction. By in situ hybridization, mRNA encoding GUCY2F is found only in the outer nuclear layer and inner segments of photoreceptor cells. By using synthetic peptide antiserum specific for each RetGC subtype, RetGC-2(GUCY2F) can be distinguished from RetGC-1 as a slightly smaller protein in immunoblots of bovine rod outer segments.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GC-F; Guanylate cyclase 2F; Guanylate cyclase 2F, retinal; Guanylate cyclase F; RETGC-2; Retinal guanylyl cyclase 2; Rod outer segment membrane guanylate cyclase 2; ROS-GC2
Gene Aliases: A930002I06; GC-F; GC2; Guc2f; Gucy2f
UniProt ID: (Mouse) Q5SDA5, (Rat) P51842
Entrez Gene ID: (Mouse) 245650, (Rat) 116556
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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