Invitrogen

PGP9.5 Monoclonal Antibody (BH7)

PGP9.5 Antibody in Immunocytochemistry (ICC/IF)

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PGP9.5 Antibody (480012) in ICC/IF

Immunofluorescence analysis of PGP9.5 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PGP9.5 Monoclonal Antibody (BH7) (Product # 480012) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product... View More

PGP9.5 Antibody in Immunohistochemistry (IHC)

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Product Details

480012

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

1:500-1:5,000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:500

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BH7

Immunogen

Recombinant full length human UCHL1 purified from E. coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein G

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2532243

Target Information

PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron specific protein, structurally and immunologically distinct from neuron specific enolase. PGP9.5 has a molecular weight of 27 kDa and was first identified by high resolution two dimensional PAGE. PGP9.5 is a member of ubiquitin carboxyl-terminal hydrolase family 1 (peptidase family C12) with a ubiquitin carboxyl-terminal hydrolase domain. PGP9.5 is well known for having ubiquitin hydrolase and ligase activities that hydrolyzes small C-terminal adducts of ubiquitin to generate ubiquitin monomers. PGP9.5 is present in neurons and nerve fibers at all levels of the central and peripheral nervous system, in neuroendocrine cells, in segments of the renal tubules, in spermatogonia and Leydig cells of the testis, in ova and in some cells of both the pregnant and non-pregnant corpus luteum. Over expression of PGP9.5 leads to non-small cell lung cancer while decreased expression leads to Huntington disease and Alzheimer disease. Since PGP9.5 is present in cellular inclusions, it can be a useful as a neuronal marker and in the studies of neurodegenerative disorders such as with Parkinson disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida.

International journal of molecular sciences

Punda H,Mardesic S,Filipovic N,Kosovic I,Benzon B,Ogorevc M,Bocina I,Kolic K,Vukojevic K,Saraga-Babic M

480012 was used in Immunohistochemistry-immunofluorescence to indicate a human-species specific sr expression pattern, and the importance of sr1 in neuronal differentiation, and sr2 and sr3 in the control of the roof plate morphogenesis in normal and disturbed development.

Wed Jul 07 00:00:00 UTC 2021

Expression of Connexins 37, 43 and 45 in Developing Human Spinal Cord and Ganglia.

International journal of molecular sciences

Jurić M,Zeitler J,Vukojević K,Bočina I,Grobe M,Kretzschmar G,Saraga-Babić M,Filipović N

480012 was used in Immunohistochemistry-immunofluorescence to characterize the expression of different types of connexins in spinal cord and dorsal root ganglia during early human developmental stages, which could imply their role in embryonal/early fetal SC development.

Tue Dec 08 00:00:00 UTC 2020

Expression of Connexins 37, 43 and 45 in Developing Human Spinal Cord and Ganglia.

International journal of molecular sciences

Jurić M,Zeitler J,Vukojević K,Bočina I,Grobe M,Kretzschmar G,Saraga-Babić M,Filipović N

Tue Dec 08 00:00:00 UTC 2020

Effects of Different n6/n3 PUFAs Dietary Ratio on Cardiac Diabetic Neuropathy.

Nutrients

Urlić M,Urlić I,Urlić H,Mašek T,Benzon B,Vitlov Uljević M,Vukojević K,Filipović N

480012 was used in Immunohistochemistry-immunofluorescence to investigate polyunsaturated fatty acid ratios in cardiac diabetic neuropathy.

Thu Sep 10 00:00:00 UTC 2020

Effects of Different n6/n3 PUFAs Dietary Ratio on Cardiac Diabetic Neuropathy.

Nutrients

Urlić M,Urlić I,Urlić H,Mašek T,Benzon B,Vitlov Uljević M,Vukojević K,Filipović N

480012 was used in Immunohistochemistry-immunofluorescence to investigate polyunsaturated fatty acid ratios in cardiac diabetic neuropathy.

Thu Sep 10 00:00:00 UTC 2020

Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes.

Nature communications

Hewings DS,Heideker J,Ma TP,AhYoung AP,El Oualid F,Amore A,Costakes GT,Kirchhofer D,Brasher B,Pillow T,Popovych N,Maurer T,Schwerdtfeger C,Forrest WF,Yu K,Flygare J,Bogyo M,Wertz IE

480012 was used in Western Blotting to evaluate the activity and probe reactivity of deubiquitinase enzymes.

Wed Mar 21 00:00:00 UTC 2018

MicroRNA-922 promotes tau phosphorylation by downregulating ubiquitin carboxy-terminal hydrolase L1 (UCHL1) expression in the pathogenesis of Alzheimer's disease.

Neuroscience

Zhao ZB,Wu L,Xiong R,Wang LL,Zhang B,Wang C,Li H,Liang L,Chen SD

480012 was used in western blot to elucidate the mechanisms that regulate UCHL1 levels in Alzheimer's disease AD.

Fri Sep 05 00:00:00 UTC 2014

Changes in epidermal thickness and cutaneous innervation during maturation in long-term diabetes.

Journal of tissue viability

Jelicic Kadic A,Boric M,Vidak M,Ferhatovic L,Puljak L

480012 was used in immunohistochemistry to examine the cutaneous changes in rats after the induction of diabetes.

Sat Feb 01 00:00:00 UTC 2014

Gender and gonadectomy influence on neurons in superior cervical ganglia of sexually mature rats.

Neuroscience letters

Filipović N,Zuvan L,Mašek T,Tokalić R,Grković I

480012 was used in immunohistochemistry to study the neuronal change in superior cervical ganglia due to sexual maturation and gonadal hormones

Thu Mar 20 00:00:00 UTC 2014

Cutaneous expression of calcium/calmodulin-dependent protein kinase II in rats with type 1 and type 2 diabetes.

Journal of chemical neuroanatomy

Boric M,Jelicic Kadic A,Puljak L

480012 was used in immunohistochemistry to analyze the expression of CaMKII in the rat foot pad 2 weeks and 2 months after induction of type 1 and 2 diabetes

Sat Nov 01 00:00:00 UTC 2014

Conformational stabilization of ubiquitin yields potent and selective inhibitors of USP7.

Nature chemical biology

Zhang Y,Zhou L,Rouge L,Phillips AH,Lam C,Liu P,Sandoval W,Helgason E,Murray JM,Wertz IE,Corn JE

480012 was used in western blot to isolate ubiquitin functional conformations that act as potent and selective USP7 inhibitors.

Tue Jan 01 00:00:00 UTC 2013

Reduced epidermal thickness, nerve degeneration and increased pain-related behavior in rats with diabetes type 1 and 2.

Journal of chemical neuroanatomy

Boric M,Skopljanac I,Ferhatovic L,Jelicic Kadic A,Banozic A,Puljak L

480012 was used in immunohistochemistry to study cutaneous changes as markers of neuropathies in two rat models of diabetes.

Fri Nov 01 00:00:00 UTC 2013

Immunofluorescent triple-staining technique to identify sensory nerve endings in human thumb ligaments.

Cells, tissues, organs

Lee J,Ladd A,Hagert E

480012 was used in immunohistochemistry (paraffin) to investigate carpometacarpal ligament innervation.

Thu Sep 20 00:00:00 UTC 2012

Ubiquitin editing enzyme UCH L1 and microtubule dynamics: implication in mitosis.

Cell cycle (Georgetown, Tex.)

Bheda A,Gullapalli A,Caplow M,Pagano JS,Shackelford J

Published figure using PGP9.5 monoclonal antibody (Product # 480012) in Western Blot

Mon Mar 01 00:00:00 UTC 2010

Ubiquitin editing enzyme UCH L1 and microtubule dynamics: implication in mitosis.

Cell cycle (Georgetown, Tex.)

Bheda A,Gullapalli A,Caplow M,Pagano JS,Shackelford J

Published figure using PGP9.5 monoclonal antibody (Product # 480012) in Western Blot

Mon Mar 01 00:00:00 UTC 2010

Ubiquitin editing enzyme UCH L1 and microtubule dynamics: implication in mitosis.

Cell cycle (Georgetown, Tex.)

Bheda A,Gullapalli A,Caplow M,Pagano JS,Shackelford J

Published figure using PGP9.5 monoclonal antibody (Product # 480012) in Western Blot

Mon Mar 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: epididymis luminal protein 117; epididymis secretory protein Li 53; gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; PGP 9.5; PGP9.5; protein gene product 9.5; ubiquitin C-terminal hydrolase; ubiquitin C-terminal hydrolase-L1; ubiquitin carboxy-terminal hydrolase L1; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal hydrolase L1; Ubiquitin thioesterase L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1; UCH-L1; UCHL

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Gene Aliases: AW822034; BOS_6576; C88048; gad; HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; R75593; UCH-L1; UCHL-1; UCHL1

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UniProt ID: (Pig) Q6SEG5, (Human) P09936, (Bovine) P23356, (Mouse) Q9R0P9, (Rat) Q00981

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Entrez Gene ID: (Pig) 396637, (Human) 7345, (Bovine) 514394, (Mouse) 22223, (Rat) 29545

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Function(s)

cysteine-type endopeptidase activity thiol-dependent ubiquitin-specific protease activity protein binding omega peptidase activity ligase activity ubiquitin protein ligase binding alpha-2A adrenergic receptor binding ubiquitin binding obsolete ubiquitin thiolesterase activity peptidase activity cysteine-type peptidase activity hydrolase activity

Process(es)

response to ischemia proteolysis ubiquitin-dependent protein catabolic process axonogenesis axon target recognition adult walking behavior cell proliferation protein deubiquitination sensory perception of pain axonal transport of mitochondrion eating behavior negative regulation of MAP kinase activity muscle fiber development neuromuscular process regulation of macroautophagy proteasome-mediated ubiquitin-dependent protein catabolic process response to stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PGP9.5 Monoclonal Antibody (BH7)

PGP9.5 Antibody (480012) in ICC/IF

Product Details

480012

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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