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Invitrogen
PHIP Polyclonal Antibody
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FIGURE: 1 / 8
PHIP Antibody (PA5-77167) in ICC/IF
Immunofluorescence analysis of PH-interacting protein (PHIP) was performed using 70% confluent log phase A-431 and U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PHIP Polyclonal Antibody (Product # PA5-77167) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plu... View More
Product Details
PA5-77167
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length Human PHIP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2720894
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
PHIP (pleckstrin homology domain interacting protein), also known as ndrp or WDR11, is a 1,821 amino acid protein that contains eight N-terminal WD40 repeats and two bromodomains. It is expressed in skeletal muscle (localizing to the cytosol and nucleus) and primary beta cells (localizing to the nucleus) and acts as a transcriptional activator. PHIP is known to interact with various members of the insulin receptor substrate (IRS) family. The IRS family of proteins mediate insulin receptor signaling and play an important role in insulin-producing beta cell proliferation and survival. PHIP specifically associates with the PH domain of IRS-1 and may function to link IRS-1 to insulin receptors, indicating a vital role of PHIP in the regulation of insulin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DDB1 and CUL4 associated factor 14; DDB1- and CUL4-associated factor 14; FLJ20705; IRS-1 PH domain-binding protein; neuronal differentiation related protein; neuronal differentiation-related protein; PH-interacting protein; PHIP; WD repeat domain 11; WD repeat-containing protein 11
Gene Aliases: 2810004D21Rik; 4632404O06Rik; BRWD2; DCAF14; Ndrp; PHIP; WDR11
UniProt ID: (Human) Q8WWQ0
Entrez Gene ID: (Human) 55023, (Mouse) 83946
regulation of protein phosphorylation
regulation of transcription from RNA polymerase II promoter
protein import into nucleus
cytoskeleton organization
positive regulation of cell proliferation
insulin receptor signaling pathway
regulation of cell shape
positive regulation of peptidyl-threonine phosphorylation
regulation of cell morphogenesis
positive regulation of peptidyl-serine phosphorylation
regulation of growth
negative regulation of apoptotic process
positive regulation of insulin-like growth factor receptor signaling pathway
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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