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PHLPPL Polyclonal Antibody

PHLPPL Antibody in Immunocytochemistry (ICC/IF)

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PHLPPL Antibody (25244-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPPL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

PHLPPL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PHLPPL Antibody in Immunoprecipitation (IP)

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Product Details

25244-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PHLPPL Fusion Protein Ag19000 (575-781 aa encoded by BC129927)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LDLQHNALT RLPDTLFSKA LNLRYLNASA NSLESLPSAC TGEESLSMLQ LLYLTNNLLT DQCIPVLVGH LHLRILHLAN NQLQTFPASK LNKLEQLEEL NLSGNKLKTI PTTIANCKRL HTLVAHSNNI SIFPEILQLP QIQFVDLSCN DLTEILIPEA LPATLQDLDL TGNTNLVLEH KTLDIFSHIT TLKIDQKPLP TTDSTVTS (575-781 aa encoded by BC129927)

Target Information

Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis (PubMed:20513427). Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation (PubMed:21986499). Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity (PubMed:24530606). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of pleckstrin homology domain and leucine-rich repeat protein phosphatase 2 has protective effects on high glucose-injured retinal ganglion cells via the effect on the Akt-GSK-3β-Nrf2 pathway.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Liu X,Liu Y,Chen L,Zhang Z,Cui L,Wei T

Wed Mar 01 00:00:00 UTC 2023

Circular RNA circRASSF5 Functions as an Anti-Oncogenic Factor in Hepatocellular Carcinoma by Acting as a Competitive Endogenous RNA Through Sponging miR-331-3p.

Journal of hepatocellular carcinoma

Zhou Z,Cui X,Gao P,Zhang X,Zhu C,Sun B

Wed Oct 12 00:00:00 UTC 2022

Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.

Frontiers in oncology

Wang W,Xia X,Chen K,Chen M,Meng Y,Lv D,Yang H

Sun Apr 24 00:00:00 UTC 2022

Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.

Frontiers in oncology

Wang W,Xia X,Chen K,Chen M,Meng Y,Lv D,Yang H

Sun Apr 24 00:00:00 UTC 2022

Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway.

Oxidative medicine and cellular longevity

Li DF,Chang X,Zhao JL,Chen XM,Xu ZL,Zhang DG,Wu BH,Wang LS,Bai Y,Yao J

Wed Jan 05 00:00:00 UTC 2022

Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway.

Oxidative medicine and cellular longevity

Li DF,Chang X,Zhao JL,Chen XM,Xu ZL,Zhang DG,Wu BH,Wang LS,Bai Y,Yao J

Wed Jan 05 00:00:00 UTC 2022

Role of epigenetic m⁶ A RNA methylation in vascular development: mettl3 regulates vascular development through PHLPP2/mTOR-AKT signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Parial R,Li H,Li J,Archacki S,Yang Z,Wang IZ,Chen Q,Xu C,Wang QK

Sat May 01 00:00:00 UTC 2021

Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.

Molecular therapy. Nucleic acids

Wang Y,Lu J,Chen L,Bian H,Hu J,Li D,Xia C,Xu H

Fri Jun 05 00:00:00 UTC 2020

Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.

Molecular therapy. Nucleic acids

Wang Y,Lu J,Chen L,Bian H,Hu J,Li D,Xia C,Xu H

Fri Jun 05 00:00:00 UTC 2020

Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.

Molecular therapy. Nucleic acids

Wang Y,Lu J,Chen L,Bian H,Hu J,Li D,Xia C,Xu H

Fri Jun 05 00:00:00 UTC 2020

Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.

Molecular therapy. Nucleic acids

Wang Y,Lu J,Chen L,Bian H,Hu J,Li D,Xia C,Xu H

Fri Jun 05 00:00:00 UTC 2020

LncRNA MBNL1-AS1 represses cell proliferation and enhances cell apoptosis via targeting miR-135a-5p/PHLPP2/FOXO1 axis in bladder cancer.

Cancer medicine

Wei X,Yang X,Wang B,Yang Y,Fang Z,Yi C,Shi L,Song D

Wed Jan 01 00:00:00 UTC 2020

miR-762 Promotes Malignant Development of Head and Neck Squamous Cell Carcinoma by Targeting PHLPP2 and FOXO4.

OncoTargets and therapy

Chen S,Zhang JY,Sun LS,Li XF,Bai JY,Zhang HY,Li TJ

Tue Apr 12 00:00:00 UTC 2022

PHLPP2 as a novel metastatic and prognostic biomarker in non-small cell lung cancer patients.

Thoracic cancer

Wang H,Gu R,Tian F,Liu Y,Fan W,Xue G,Cai L,Xing Y

Fri Nov 01 00:00:00 UTC 2019

High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Oncotarget

Xie Y,Lv D,Wang W,Ye M,Chen X,Yang H

Tue Aug 29 00:00:00 UTC 2017

High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Oncotarget

Xie Y,Lv D,Wang W,Ye M,Chen X,Yang H

Tue Aug 29 00:00:00 UTC 2017

High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Oncotarget

Xie Y,Lv D,Wang W,Ye M,Chen X,Yang H

Tue Aug 29 00:00:00 UTC 2017

High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.

Oncotarget

Xie Y,Lv D,Wang W,Ye M,Chen X,Yang H

Tue Aug 29 00:00:00 UTC 2017

PHLPP regulates hexokinase 2-dependent glucose metabolism in colon cancer cells.

Cell death discovery

Xiong X,Wen YA,Mitov MI,C Oaks M,Miyamoto S,Gao T

Wed Sep 30 00:00:00 UTC 2020

PHLPP regulates hexokinase 2-dependent glucose metabolism in colon cancer cells.

Cell death discovery

Xiong X,Wen YA,Mitov MI,C Oaks M,Miyamoto S,Gao T

Wed Sep 30 00:00:00 UTC 2020

Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.

The Journal of biological chemistry

Xiong X,Li X,Wen YA,Gao T

Fri Nov 25 00:00:00 UTC 2016

Pleckstrin Homology (PH) Domain Leucine-rich Repeat Protein Phosphatase Controls Cell Polarity by Negatively Regulating the Activity of Atypical Protein Kinase C.

The Journal of biological chemistry

Xiong X,Li X,Wen YA,Gao T

Fri Nov 25 00:00:00 UTC 2016

PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.

Oncotarget

Smith AJ,Wen YA,Stevens PD,Liu J,Wang C,Gao T

Tue Feb 16 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: GTPase activating protein and VPS9 domains 1; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like; PHLPP-like; protein phosphatase, Mg2+/Mn2+ dependent 3B

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Gene Aliases: AI481772; C130044A18Rik; KIAA0931; PHLPP2; PHLPPL; PPM3B; RGD1562857

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UniProt ID: (Human) Q6ZVD8, (Mouse) Q8BXA7

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Entrez Gene ID: (Human) 23035, (Mouse) 244650, (Rat) 498949

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Function(s)

protein serine/threonine phosphatase activity metal ion binding catalytic activity phosphoprotein phosphatase activity hydrolase activity

Process(es)

protein dephosphorylation hippocampus development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PHLPPL Polyclonal Antibody

PHLPPL Antibody (25244-1-AP) in ICC/IF

Product Details

25244-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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