Proteintech

PHOX2B Polyclonal Antibody

PHOX2B Antibody in Immunocytochemistry (ICC/IF)

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PHOX2B Antibody (25276-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 25276-1-AP (PHOX2B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

PHOX2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Product Details

25276-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PHOX2B Fusion Protein Ag13196 (63-314 aa encoded by BC017199)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GSCSLGTLR DHQSSPYAAV PYKLFTDHGG LNEKRKQRRI RTTFTSAQLK ELERVFAETH YPDIYTREEL ALKIDLTEAR VQVWFQNRRA KFRKQERAAA AAAAAAKNGS SGKKSDSSRD DESKEAKSTD PDSTGGPGPN PNPTPSCGAN GGGGGGPSPA GAPGAAGPGG PGGEPGKGGA AAAAAAAAAA AAAAAAAAAG GLAAAGGPGQ GWAPGPGPIT SIPDSLGGPF ASVLSSLQRP NGAKAALVKS SMF (63-314 aa encoded by BC017199)

Target Information

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deciphering the Molecular Mechanisms of Autonomic Nervous System Neuron Induction through Integrative Bioinformatics Analysis.

International journal of molecular sciences

Takayama Y,Akagi Y,Kida YS

Sun May 21 00:00:00 UTC 2023

Neural Stem Cell-Derived Exosomal Netrin1 Contributes to Neuron Differentiation of Mesenchymal Stem Cells in Therapy of Spinal Bifida Aperta.

Stem cells translational medicine

Ma L,Wei X,Ma W,Liu Y,Wang Y,He Y,Jia S,Wang Y,Luo W,Liu D,Huang T,Yan J,Gu H,Bai Y,Yuan Z

Fri May 27 00:00:00 UTC 2022

Selective Induction of Human Autonomic Neurons Enables Precise Control of Cardiomyocyte Beating.

Scientific reports

Takayama Y,Kushige H,Akagi Y,Suzuki Y,Kumagai Y,Kida YS

Thu Jun 11 00:00:00 UTC 2020

Generating trunk neural crest from human pluripotent stem cells.

Scientific reports

Huang M,Miller ML,McHenry LK,Zheng T,Zhen Q,Ilkhanizadeh S,Conklin BR,Bronner ME,Weiss WA

Wed Jan 27 00:00:00 UTC 2016

Generating trunk neural crest from human pluripotent stem cells.

Scientific reports

Huang M,Miller ML,McHenry LK,Zheng T,Zhen Q,Ilkhanizadeh S,Conklin BR,Bronner ME,Weiss WA

Wed Jan 27 00:00:00 UTC 2016

Ablation of intact hypothalamic and/or hindbrain TrkB signaling leads to perturbations in energy balance.

Molecular metabolism

Ozek C,Zimmer DJ,De Jonghe BC,Kalb RG,Bence KK

Sun Nov 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: dilated pupils 1; GENA 269; NBPhox; neuroblastoma paired-type homeobox protein; Neuroblastoma Phox; paired mesoderm homeobox 2b; Paired mesoderm homeobox protein 2B; Paired-like homeobox 2B; PHOX2B homeodomain protein

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Gene Aliases: Dilp1; NBLST2; NBPhox; PHOX2B; PMX2B

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UniProt ID: (Human) Q99453, (Mouse) O35690

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Entrez Gene ID: (Human) 8929, (Mouse) 18935

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding sequence-specific DNA binding

Process(es)

neuron migration regulation of respiratory gaseous exchange by neurological system process noradrenergic neuron differentiation noradrenergic neuron development brainstem development transcription from RNA polymerase II promoter negative regulation of cell proliferation glial cell differentiation regulation of gene expression positive regulation of G2/M transition of mitotic cell cycle cell differentiation in hindbrain medullary reticular formation development hindbrain tangential cell migration skeletal muscle cell differentiation negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter autonomic nervous system development enteric nervous system development sympathetic nervous system development parasympathetic nervous system development inner ear development efferent axon development in a lateral line nerve respiratory system development retrotrapezoid nucleus neuron differentiation sympathetic ganglion development negative regulation of cell cycle arrest dopaminergic neuron differentiation cellular response to BMP stimulus neural crest cell migration involved in autonomic nervous system development regulation of transcription, DNA-templated multicellular organism development neuron differentiation cell development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PHOX2B Polyclonal Antibody

PHOX2B Antibody (25276-1-AP) in ICC/IF

Product Details

25276-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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