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Invitrogen
PI3 Polyclonal Antibody
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FIGURE: 1 / 5
PI3 Antibody (PA5-79818) in ICC/IF
Immunocytochemistry analysis of Elafin/Skalp using antiElafin/Skalp antibody (Product # PA5-79818) . Elafin/Skalp was detected in a section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Elafin/Skalp antibody (Product # PA5-79818) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was ... View More
Product Details
PA5-79818
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746933
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue. IHC: human tonsil tissue. ICC/IF: A549 cell. Flow: SiHa cell.
Target Information
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Elafin; Elastase-specific inhibitor; ESI; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; Skin-derived antileukoproteinase; trappin-2; WAP four-disulfide core domain 14; WAP four-disulfide core domain protein 14
Gene Aliases: cementoin; ESI; PI3; SKALP; WAP3; WFDC14
UniProt ID: (Human) E1P618
Entrez Gene ID: (Human) 5266
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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