Invitrogen

PI3K p85 alpha Monoclonal Antibody (6G10)

View all (28) PI3K p85 alpha antibodies

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Datasheet
Protocols
Questions & Answers

Product Details

MA5-17150

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6G10

Immunogen

Purified recombinant fragment of human PIK3R1 (amino acids: 159-388) expressed in E. Coli.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538621

Product Specific Information

MA5-17150 targets PIK3R1 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17150 immunogen is purified recombinant fragment of human PIK3R1 (amino acids: 159-388) expressed in E. Coli.

MA5-17150 detects PIK3R1 which has a predicted molecular weight of approximately 83.6kDa.

Target Information

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: p55-GAMMA; p55PIK; phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase p85-subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; polypeptide 1 p85 alpha; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-al; RP4-533D7.2

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Gene Aliases: AGM7; GRB1; IMD36; p50alpha; p55alpha; p85; p85-ALPHA; p85alpha; PI3K; PIK3R1

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UniProt ID: (Human) P27986, (Mouse) P26450

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Entrez Gene ID: (Human) 5295, (Mouse) 18708

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Function(s)
transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity receptor binding platelet-derived growth factor receptor binding calmodulin binding protein C-terminus binding protein kinase binding protein domain specific binding estrogen receptor binding receptor tyrosine kinase binding ubiquitin protein ligase binding ATPase binding phosphoprotein binding
Process(es)
cellular glucose homeostasis positive regulation of protein phosphorylation negative regulation of cell-matrix adhesion glucose metabolic process protein phosphorylation transport negative regulation of cell adhesion signal transduction insulin receptor signaling pathway extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of heart rate positive regulation of gene expression negative regulation of muscle cell apoptotic process phosphatidylinositol 3-kinase signaling protein transport negative regulation of cell-cell adhesion B cell differentiation positive regulation of cell migration positive regulation of tumor necrosis factor production response to insulin cellular response to insulin stimulus positive regulation of RNA splicing cellular response to UV response to endoplasmic reticulum stress phosphatidylinositol-3-phosphate biosynthetic process positive regulation of transcription factor import into nucleus negative regulation of apoptotic process regulation of phosphatidylinositol 3-kinase activity positive regulation of myoblast differentiation negative regulation of osteoclast differentiation negative regulation of blood pressure negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway negative regulation of smooth muscle cell proliferation protein stabilization response to glucocorticoid regulation of stress fiber assembly NFAT protein import into nucleus response to cAMP growth hormone receptor signaling pathway regulation of establishment of protein localization to plasma membrane positive regulation of endoplasmic reticulum unfolded protein response phosphatidylinositol biosynthetic process epidermal growth factor receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling viral process platelet activation T cell costimulation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway positive regulation of glucose import vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling T cell receptor signaling pathway leukocyte migration positive regulation of establishment of protein localization to plasma membrane positive regulation of glucose import in response to insulin stimulus
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