Invitrogen

PI3K p85/p55 Recombinant Rabbit Monoclonal Antibody (10L6B7)

View all (13) PI3K p85/p55 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-51324

Applications
Tested Dilution
Publications

Western Blot (WB)

1:4,500-1:18,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

10L6B7

Immunogen

A synthetic peptide of human PI3 Kinase p85 alpha/beta (NP_852664.1).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3094402

Product Specific Information

Immunogen sequence: RLYEE

Target Information

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000009783; OTTHUMP00000009784; p55PIK; phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55); Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase p55 subunit; phosphatidylinositol 3-kinase regulartory subunit polypeptide 2; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulartory subunit, polypeptide 2; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50); Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); PI3-kinase p85 subunit alpha; PI3-kinase p85 subunit beta; PI3-kinase p85 subunit gamma; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit beta; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3-kinase subunit p85-beta; PI3K regulatory subunit alpha; PI3K regulatory subunit beta; PI3K regulatory subunit gamma; polypeptide 3; ptdIns-3-kinase p85-alpha; ptdIns-3-kinase p85-beta; ptdIns-3-kinase p85-gamma; ptdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase regulatory subunit p85-al; ptdIns-3-kinase regulatory subunit p85-alpha; ptdIns-3-kinase regulatory subunit p85-beta; RP4-533D7.2

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Gene Aliases: AA414954; p50alpha; p55alpha; p55pik; p85alpha; p85beta; PI3K; PI3KA; Pik3r1; Pik3r2; Pik3r3

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UniProt ID: (Mouse) P26450, (Rat) Q63787, (Mouse) O08908, (Rat) Q63789, (Mouse) Q64143

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Entrez Gene ID: (Mouse) 18708, (Rat) 25513, (Mouse) 18709, (Rat) 29741, (Rat) 60664, (Mouse) 18710

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Function(s)
receptor binding insulin receptor binding insulin-like growth factor receptor binding platelet-derived growth factor receptor binding neurotrophin TRKA receptor binding protein binding calmodulin binding protein C-terminus binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein kinase binding protein phosphatase binding protein domain specific binding estrogen receptor binding receptor tyrosine kinase binding ubiquitin protein ligase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity ATPase binding phosphoprotein binding kinase activity
Process(es)
insulin receptor signaling pathway phosphorylation regulation of phosphatidylinositol 3-kinase activity cellular glucose homeostasis positive regulation of protein phosphorylation negative regulation of cell-matrix adhesion glucose metabolic process protein phosphorylation transport negative regulation of cell adhesion signal transduction extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of heart rate positive regulation of gene expression negative regulation of muscle cell apoptotic process phosphatidylinositol 3-kinase signaling protein transport negative regulation of cell-cell adhesion B cell differentiation positive regulation of cell migration positive regulation of tumor necrosis factor production response to insulin cellular response to insulin stimulus positive regulation of RNA splicing cellular response to UV response to endoplasmic reticulum stress phosphatidylinositol-3-phosphate biosynthetic process positive regulation of transcription factor import into nucleus negative regulation of apoptotic process positive regulation of myoblast differentiation negative regulation of osteoclast differentiation negative regulation of blood pressure negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway negative regulation of smooth muscle cell proliferation protein stabilization response to glucocorticoid regulation of stress fiber assembly NFAT protein import into nucleus response to cAMP growth hormone receptor signaling pathway regulation of establishment of protein localization to plasma membrane positive regulation of endoplasmic reticulum unfolded protein response regulation of autophagy regulation of actin filament polymerization regulation of phosphorylation positive regulation of cell adhesion response to yeast aging response to nutrient response to fructose response to iron(II) ion response to estradiol response to progesterone response to testosterone response to drug response to amino acid response to ethanol response to fatty acid response to growth factor cellular response to fatty acid response to dexamethasone
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