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Rockland
PIAS1 Polyclonal Antibody
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FIGURE: 1 / 1
PIAS1 Antibody (600-401-GZ8) in WB
Western Blot of PIAS1 antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: HEK293 whole cell lysate p/n (W09-000-365). Lane 3: K-562 whole cell lysate p/n (W09-001-GJ7). Primary Antibody: Anti-PIAS1 antibody at 1.0 µg/mL. Secondary antibody: Peroxidase rabbit secondary antibody p/n (611-103-122) at 1:70,000 for 45 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 72 kDa, 60 kDa for PIAS1. Other band(s): PIAS1 splice variants and isoforms.
Product Details
600-401-GZ8
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-PIAS1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the internal region of human PIAS1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
A BLAST analysis was used to suggest reactivity to mouse 92.9% homology for the immunogen sequence. Cross-reactivity from PIAS1 family and other sources has not been determined.
Target Information
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AR interacting protein; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; E3 SUMO-protein transferase PIAS1; GBP; Gu binding protein; Gu-binding protein; Protein inhibitor of activated STAT protein 1; protein inhibitor of activated STAT-1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3
Gene Aliases: DDXBP1; GBP; GU/RH-II; PIAS1; ZMIZ3
UniProt ID: (Human) O75925
Entrez Gene ID: (Human) 8554
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
JAK-STAT cascade
protein sumoylation
androgen receptor signaling pathway
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of protein sumoylation
regulation of cell proliferation
fat cell differentiation
positive regulation of transcription, DNA-templated
regulation of interferon-gamma-mediated signaling pathway
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