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Proteintech
PIAS3 Polyclonal Antibody
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Product Details
13486-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
PIAS3 Fusion Protein Ag4304 (277-628 aa encoded by BC030556)
Conjugate
Form
Liquid
Concentration
0.23 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
The PIAS3 antibody detects PIAS3 (68 kDa) as well as its sumoylated form (85 kDa) in some normal mouse tissues, breast and brain tumor cell lines.
Immunogen sequence: VRQLTAGTL LQKLRAKGIR NPDHSRALIK EKLTADPDSE VATTSLRVSL MCPLGKMRLT VPCRALTCAH LQSFDAALYL QMNEKKPTWT CPVCDKKAPY ESLIIDGLFM EILSSCSDCD EIQFMEDGSW CPMKPKKEAS EVCPPPGYGL DGLQYSPVQG GDPSENKKKV EVIDLTIESS SDEEDLPPTK KHCSVTSAAI PALPGSKGVL TSGHQPSSVL RSPAMGTLGG DFLSSLPLHE YPPAFPLGAD IQGLDLFSFL QTESQHYGPS VITSLDEQDA LGHFFQYRGT PSHFLGPLAP TLGSSHCSAT PAPPPGRVSS IVAPGGALRE GHGGPLPSGP SLTGCRSDII SLD (277-628 aa encoded by BC030556)
Target Information
The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 SUMO-protein ligase PIAS3; E3 SUMO-protein transferase PIAS3; KChAP; potassium channel regulatory protein KChAP; Potassium channel-associated protein; Protein inhibitor of activated STAT protein 3; protein inhibitor of activated STAT, 3; zinc finger, MIZ-type containing 5
Gene Aliases: PIAS3; Pias3l; ZMIZ5
UniProt ID: (Human) Q9Y6X2, (Mouse) O54714, (Rat) O70260
Entrez Gene ID: (Human) 10401, (Mouse) 229615, (Rat) 83614
protein binding
protein C-terminus binding
zinc ion binding
potassium channel regulator activity
ligase activity
SUMO transferase activity
enzyme binding
protein N-terminus binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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