Proteintech

PIAS4 Polyclonal Antibody

FIGURE: 1 / 4

PIAS4 Antibody (14242-1-AP) in WB

Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Product Details

14242-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIAS4 Fusion Protein Ag5498 (210-510 aa encoded by BC029874)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PNIAVKVNH SYCSVPGYYP SNKPGVEPKR PCRPINLTHL MYLSSATNRI TVTWGNYGKS YSVALYLVRQ LTSSELLQRL KTIGVKHPEL CKALVKEKLR LDPDSEIATT GVRVSLICPL VKMRLSVPCR AETCAHLQCF DAVFYLQMNE KKPTWMCPVC DKPAPYDQLI IDGLLSKILS ECEDADEIEY LVDGSWCPIR AEKERSCSPQ GAILVLGPSD ANGLLPAPSV NGSGALGSTG GGGPVGSMEN GKPGADVVDL TLDSSSSSED EEEEEEEEED EDEEGPRPKR RCPFQKGLVP AC (210-510 aa encoded by BC029874)

Target Information

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy.

Nature communications

Jin S,He X,Ma L,Zhuang Z,Wang Y,Lin M,Cai S,Wei L,Wang Z,Zhao Z,Wu Y,Sun L,Li C,Xie W,Zhao Y,Songyang Z,Peng K,Zhao J,Cui J

Sat Sep 03 00:00:00 UTC 2022

Blockade of CBX4-mediated β-catenin SUMOylation attenuates airway epithelial barrier dysfunction in asthma.

International immunopharmacology

Liang S,Zhou Z,Zhou Z,Liang J,Lin W,Zhang C,Zhou C,Zhao H,Meng X,Zou F,Yu C,Cai S

Thu Dec 01 00:00:00 UTC 2022

Hengshun Aromatic Vinegar Ameliorates Vascular Endothelial Injury via Regulating PKCζ-Mediated Oxidative Stress and Apoptosis.

Frontiers in nutrition

Li X,Gao M,Zhu S,Yin L,Zhang B,Qi Y,Zhao Y,Yu Y,Xu L

Tue Jun 15 00:00:00 UTC 2021

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Nature communications

Barroso-Gomila O,Trulsson F,Muratore V,Canosa I,Merino-Cacho L,Cortazar AR,Pérez C,Azkargorta M,Iloro I,Carracedo A,Aransay AM,Elortza F,Mayor U,Vertegaal ACO,Barrio R,Sutherland JD

Thu Nov 18 00:00:00 UTC 2021

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Nature communications

Barroso-Gomila O,Trulsson F,Muratore V,Canosa I,Merino-Cacho L,Cortazar AR,Pérez C,Azkargorta M,Iloro I,Carracedo A,Aransay AM,Elortza F,Mayor U,Vertegaal ACO,Barrio R,Sutherland JD

Thu Nov 18 00:00:00 UTC 2021

DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.

PLoS biology

Yan YL,Zhang C,Hao J,Wang XL,Ming J,Mi L,Na J,Hu X,Wang Y

Sat Jun 01 00:00:00 UTC 2019

DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.

PLoS biology

Yan YL,Zhang C,Hao J,Wang XL,Ming J,Mi L,Na J,Hu X,Wang Y

Sat Jun 01 00:00:00 UTC 2019

Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.

Nature communications

Despras E,Sittewelle M,Pouvelle C,Delrieu N,Cordonnier AM,Kannouche PL

Fri Nov 04 00:00:00 UTC 2016

Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.

Nature communications

Despras E,Sittewelle M,Pouvelle C,Delrieu N,Cordonnier AM,Kannouche PL

Fri Nov 04 00:00:00 UTC 2016

Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.

PloS one

Torikoshi K,Abe H,Matsubara T,Hirano T,Ohshima T,Murakami T,Araki M,Mima A,Iehara N,Fukatsu A,Kita T,Arai H,Doi T

Fri Dec 07 00:00:00 UTC 2012

Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.

PloS one

Torikoshi K,Abe H,Matsubara T,Hirano T,Ohshima T,Murakami T,Araki M,Mima A,Iehara N,Fukatsu A,Kita T,Arai H,Doi T

Fri Dec 07 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: e3 sumo- ligase pias4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIASy; protein inhibitor of activated STAT PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; zinc finger, MIZ-type containing 6

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Gene Aliases: PIAS-gamma; PIAS4; PIASG; PIASY; ZMIZ6

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UniProt ID: (Human) Q8N2W9, (Mouse) Q9JM05

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Entrez Gene ID: (Human) 51588, (Rat) 362827, (Mouse) 59004

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Function(s)

DNA binding transcription corepressor activity protein binding protein C-terminus binding zinc ion binding ligase activity SUMO transferase activity ubiquitin protein ligase binding SUMO ligase activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter biological_process metabolic process protein sumoylation negative regulation of NF-kappaB transcription factor activity positive regulation of protein sumoylation negative regulation of transcription, DNA-templated positive regulation of keratinocyte apoptotic process positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage transcription, DNA-templated regulation of transcription, DNA-templated JAK-STAT cascade multicellular organism development negative regulation of tumor necrosis factor-mediated signaling pathway viral process Wnt signaling pathway negative regulation of nucleic acid-templated transcription double-strand break repair via nonhomologous end joining

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PIAS4 Polyclonal Antibody

PIAS4 Antibody (14242-1-AP) in WB

Product Details

14242-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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