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Invitrogen
PIASy Polyclonal Antibody
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FIGURE: 1 / 3
PIASy Antibody (PA5-30628) in ICC/IF
PIASy Polyclonal Antibody detects PIAS4 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PIAS4 protein stained by PIASy Polyclonal Antibody (Product # PA5-30628) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-30628
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PIASy. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2548102
Product Specific Information
Recommended positive controls: human PIAS4-transfected 293T.
Predicted reactivity: Chimpanzee (97%), Bovine (87%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: e3 sumo- ligase pias4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; zinc finger, MIZ-type containing 6
Gene Aliases: PIAS-gamma; PIAS4; PIASG; PIASY; ZMIZ6
UniProt ID: (Human) Q8N2W9
Entrez Gene ID: (Human) 51588
negative regulation of transcription from RNA polymerase II promoter
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
negative regulation of tumor necrosis factor-mediated signaling pathway
Wnt signaling pathway
protein sumoylation
negative regulation of NF-kappaB transcription factor activity
positive regulation of protein sumoylation
negative regulation of transcription, DNA-templated
positive regulation of keratinocyte apoptotic process
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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