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Invitrogen
PIASy Polyclonal Antibody
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PIASy Antibody (PA5-99455) in IHC (P)
Immunohistochemistry analysis of PIASy in mouse colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PIASy polyclonal antibody (Product # PA5-99455) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-99455
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PIAS4(Accession Q8N2W9), corresponding to amino acid residues L468-C510.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818388
Product Specific Information
Antibody detects endogenous levels of total PIAS4.
Target Information
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: e3 sumo- ligase pias4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIASy; protein inhibitor of activated STAT PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; zinc finger, MIZ-type containing 6
Gene Aliases: PIAS-gamma; PIAS4; PIASG; PIASY; ZMIZ6
UniProt ID: (Human) Q8N2W9, (Mouse) Q9JM05
Entrez Gene ID: (Human) 51588, (Rat) 362827, (Mouse) 59004
DNA binding
transcription corepressor activity
protein binding
protein C-terminus binding
zinc ion binding
ligase activity
SUMO transferase activity
ubiquitin protein ligase binding
SUMO ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
biological_process
metabolic process
protein sumoylation
negative regulation of NF-kappaB transcription factor activity
positive regulation of protein sumoylation
negative regulation of transcription, DNA-templated
positive regulation of keratinocyte apoptotic process
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
transcription, DNA-templated
regulation of transcription, DNA-templated
JAK-STAT cascade
multicellular organism development
negative regulation of tumor necrosis factor-mediated signaling pathway
viral process
Wnt signaling pathway
negative regulation of nucleic acid-templated transcription
double-strand break repair via nonhomologous end joining
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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