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Invitrogen
PIEZO1 Recombinant Mouse Monoclonal Antibody (2-10-R-A)
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FIGURE: 1 / 5
PIEZO1 Antibody (MA5-50642) in ICC/IF
Immunocytochemistry analysis of PIEZO1 in A431 cells. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with PIEZO1 recombinant monoclonal antibody (Product # MA5-50642) with a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was labelled in blue wi... View More
Product Details
MA5-50642
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2-10-R-A
Immunogen
Recombinant protein within Human Protein FAM38A aa 1275-1540/2521.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3094150
Product Specific Information
Positive control: Recombinant protein lysates, rat brain tissue, mouse hippocampus tissue, mouse brain tissue.
Predicted band size: 287 kDa
Subcellular Location: Endoplasmic reticulum membrane Cell membrane.
Target Information
Piezo1 is a pore-forming subunit of a mechanosensitive non-specific cation channel, and generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Structurally, Piezo1 contains 36 transmembrane domains and functions as a homotetramer. Piezo1 plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, Piezo1 may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Piezo1 acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation and plays a key role in blood vessel formation and vascular structure in both development and adult physiology. The protein encoded by the Piezo1 gene is a mechanically-activated ion channel that links mechanical forces to biological signals. Defects in this gene have been associated with dehydrated hereditary stomatocytosis. Diseases associated with PIEZO1 include Dehydrated Hereditary Stomatocytosis with/without Pseudohyperkalemia And/Or Perinatal Edema and Lymphedema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: family with sequence similarity 38, member A; Membrane protein induced by beta-amyloid treatment; Mib; Piezo-type mechanosensitive ion channel component 1; Protein FAM38A; protein PIEZO1
Gene Aliases: 9630020g22; DHS; FAM38A; KIAA0233; LMPH3; Mib; mKIAA0233; PIEZO1
UniProt ID: (Human) Q92508, (Rat) Q0KL00
Entrez Gene ID: (Human) 9780, (Rat) 361430, (Mouse) 234839
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