Proteintech

PIK3C2A Polyclonal Antibody

PIK3C2A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

PIK3C2A Antibody (22028-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 22028-1-AP (PIK3C2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PIK3C2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PIK3C2A Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22028-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIK3C2A Fusion Protein Ag16894 (1-338 aa encoded by BC113658)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAQISSNSG FKECPSSHPE PTRAKDVDKE EALQMEAEAL AKLQKDRQVT DNQRGFELSS STRKKAQVYN KQDYDLMVFP ESDSQKRALD IDVEKLTQAE LEKLLLDDSF ETKKTPVLPV TPILSPSFSA QLYFRPTIQR GQWPPGLPGP STYALPSIYP STYSKQAAFQ NGFNPRMPTF PSTEPIYLSL PGQSPYFSYP LTPATPFHPQ GSLPIYRPVV STDMAKLFDK IASTSEFLKN GKARTDLEIT DSKVSNLQVS PKSEDISKFD WLDLDPLSKP KVDNVEVLDH EEEKNVSSLL AKDPWDAVLL EERSTANCHL ERKVNGKSLS VATVTRSQS (1-338 aa encoded by BC113658 )

Target Information

The Class II PI3 kinases, including PIK3C2A, phosphorylate the 3 position of PI or PI4P. Though not as well studied as the Class I PI3 kinases, the Class II enyzmes have been linked to diverse, receptor-mediated cellular processes including insulin signaling, neuronal survival, and growth factor signaling. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Kinase-independent synthesis of 3-phosphorylated phosphoinositides by a phosphotransferase.

Nature cell biology

Walpole GFW,Pacheco J,Chauhan N,Clark J,Anderson KE,Abbas YM,Brabant-Kirwan D,Montaño-Rendón F,Liu Z,Zhu H,Brumell JH,Deiters A,Stephens LR,Hawkins PT,Hammond GRV,Grinstein S,Fairn GD

Sun May 01 00:00:00 UTC 2022

Phosphoinositide Conversion Inactivates R-RAS and Drives Metastases in Breast Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Li H,Prever L,Hsu MY,Lo WT,Margaria JP,De Santis MC,Zanini C,Forni M,Novelli F,Pece S,Di Fiore PP,Porporato PE,Martini M,Belabed H,Nazare M,Haucke V,Gulluni F,Hirsch E

Tue Mar 01 00:00:00 UTC 2022

Phosphoinositide Conversion Inactivates R-RAS and Drives Metastases in Breast Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Li H,Prever L,Hsu MY,Lo WT,Margaria JP,De Santis MC,Zanini C,Forni M,Novelli F,Pece S,Di Fiore PP,Porporato PE,Martini M,Belabed H,Nazare M,Haucke V,Gulluni F,Hirsch E

Tue Mar 01 00:00:00 UTC 2022

PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.

Science (New York, N.Y.)

Gulluni F,Prever L,Li H,Krafcikova P,Corrado I,Lo WT,Margaria JP,Chen A,De Santis MC,Cnudde SJ,Fogerty J,Yuan A,Massarotti A,Sarijalo NT,Vadas O,Williams RL,Thelen M,Powell DR,Schueler M,Wiesener MS,Balla T,Baris HN,Tiosano D,McDermott BM,Perkins BD,Ghigo A,Martini M,Haucke V,Boura E,Merlo GR,Buchner DA,Hirsch E

Fri Dec 10 00:00:00 UTC 2021

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.

Journal of cell science

Ryan EL,Shelford J,Massam-Wu T,Bayliss R,Royle SJ

Mon Feb 01 00:00:00 UTC 2021

Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.

Journal of cell science

Ryan EL,Shelford J,Massam-Wu T,Bayliss R,Royle SJ

Mon Feb 01 00:00:00 UTC 2021

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Frontiers in cellular and infection microbiology

Li T,Cui L,Xu X,Zhang H,Jiang Y,Ren L,Yang C,Liu X,Hu X

Fri Jun 18 00:00:00 UTC 2021

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Cell research

Zhang SX,Duan LH,He SJ,Zhuang GF,Yu X

Wed Feb 01 00:00:00 UTC 2017

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Cell research

Zhang SX,Duan LH,He SJ,Zhuang GF,Yu X

Wed Feb 01 00:00:00 UTC 2017

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Cell research

Zhang SX,Duan LH,He SJ,Zhuang GF,Yu X

Wed Feb 01 00:00:00 UTC 2017

Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.

Cell research

Zhang SX,Duan LH,He SJ,Zhuang GF,Yu X

Wed Feb 01 00:00:00 UTC 2017

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Cancer cell

Gulluni F,Martini M,De Santis MC,Campa CC,Ghigo A,Margaria JP,Ciraolo E,Franco I,Ala U,Annaratone L,Disalvatore D,Bertalot G,Viale G,Noatynska A,Compagno M,Sigismund S,Montemurro F,Thelen M,Fan F,Meraldi P,Marchiò C,Pece S,Sapino A,Chiarle R,Di Fiore PP,Hirsch E

Mon Oct 09 00:00:00 UTC 2017

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Cancer cell

Mon Oct 09 00:00:00 UTC 2017

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Cancer cell

Mon Oct 09 00:00:00 UTC 2017

Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.

Cancer cell

Mon Oct 09 00:00:00 UTC 2017

A four-gene signature predicts survival in clear-cell renal-cell carcinoma.

Oncotarget

Dai J,Lu Y,Wang J,Yang L,Han Y,Wang Y,Yan D,Ruan Q,Wang S

Tue Dec 13 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: C2-containing phosphatidylinositol kinase; Cpk-m; DKFZp686L193; MGC142218; p170; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha; Phosphoinositide 3-kinase-C2-alpha; phosphoinositide-3-kinase, class 2, alpha polypeptide; PI3K-C2-alpha; pi3kc2a; ptdIns-3-kinase C2 subunit alpha

View more View less

Gene Aliases: CPK; Cpk-m; PI3-K-C2(ALPHA); PI3-K-C2A; PI3K-C2-alpha; PI3K-C2alpha; PI3KC2; PIK3C2A

View more View less

UniProt ID: (Human) O00443, (Mouse) Q61194

View more View less

Entrez Gene ID: (Human) 5286, (Mouse) 18704

View more View less

Function(s)

ATP binding 1-phosphatidylinositol-3-kinase activity phosphatidylinositol 3-kinase activity 1-phosphatidylinositol-4-phosphate 3-kinase activity phosphatidylinositol binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor

Process(es)

adaptive immune response protein phosphorylation exocytosis endocytosis inflammatory response phosphatidylinositol 3-kinase signaling phosphorylation platelet activation phosphatidylinositol-3-phosphate biosynthetic process innate immune response phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling clathrin coat assembly cell chemotaxis negative regulation of zinc ion transmembrane transport phosphatidylinositol biosynthetic process epidermal growth factor receptor signaling pathway insulin receptor signaling pathway vascular smooth muscle contraction platelet-derived growth factor receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

PIK3C2A Polyclonal Antibody

PIK3C2A Antibody (22028-1-AP) in ICC/IF

Product Details

22028-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!