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Invitrogen
PIM1 Polyclonal Antibody
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FIGURE: 1 / 2
PIM1 Antibody (PA5-89681) in WB
Western blot analysis of PIM1 in extracts of various cell lines. Samples were incubated with PIM1 Polyclonal antibody (Product # PA5-89681) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-89681
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PIM1 (NP_0012301151)
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805726
Product Specific Information
Positive Samples: H460, Mouse kidney, Rat kidney; Cellular Location: Cell membrane, Cytoplasm, Nucleus
Immunogen sequence: MPHEPHEPLT PPFSALPDPA GAPSRRQSRQ RPQLSSDSPS AFRASRSHSR NATRSHSHSH SPRHSLRHSP GSGSCGSSSG HRPCADILEV GMLLSKINSL AHLRAAPCND LHATKLAPGK EKEPLESQYQ VGPLLGSGGF GSVYSGIRVS DNLPVAIKHV EKDRISDWGE LPNGTRVPME VVLLKKVSSG FSGVIRLLDW FERPDSFVLI LERPEPVQDL FDFITERGAL QEELARSFFW QVLEAVRHCH NCGVLHRDIK DENILIDLNR GELKLIDFGS GALLKDTVYT DFDGTRVYSP PEWIRYHRYH GRS
Target Information
The proto-oncogene PIM1 is upregulated in prostate cancer. PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: non-specific serine/threonine protein kinase; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase Pim-1; proviral integration site 1; Serine/threonine-protein kinase pim-1
Gene Aliases: PIM; Pim-1; PIM1
UniProt ID: (Human) P11309, (Rat) P26794, (Mouse) P06803
Entrez Gene ID: (Human) 5292, (Rat) 24649, (Mouse) 18712
protein phosphorylation
apoptotic process
cell cycle
multicellular organism development
cell proliferation
hyaluronan metabolic process
negative regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
protein autophosphorylation
vitamin D receptor signaling pathway
regulation of mitotic cell cycle
positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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