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Invitrogen
PIN1 Monoclonal Antibody (5E5)
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FIGURE: 1 / 3
PIN1 Antibody (MA5-49154) in ICC/IF
Immunocytochemistry analysis of PIN1 in Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in PIN1 Monoclonal antibody (Product # MA5-49154) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for t... View More
Product Details
MA5-49154
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
5E5
Immunogen
E.coli-derived human Pin1 recombinant protein (Position: M1-E163).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074812
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Human Pin1 shares 95% amino acid (aa) sequence identity with mouse Pin1.
Positive Control - WB: human Hela whole cell, human HEK293 whole cell, human SW620 whole cell, monkey COS-7 whole cell, rat brain tissue, rat C6 whole cell. ICC/IF: Hela cell. Flow: U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
PIN1 (Peptidylprolyl Cis/Trans Isomerase, NIMA-Interacting 1) is a Protein Coding gene. Diseases associated with PIN1 include Meibomian Cyst and Viral Exanthem. Among its related pathways are Transport of the SLBP independent Mature mRNA and Cytokine Signaling in Immune system. Gene Ontology (GO) annotations related to this gene include peptidyl-prolyl cis-trans isomerase activity and mitogen-activated protein kinase binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 5.2.1.8; NIMA-interacting; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; Rotamase Pin1
Gene Aliases: DOD; PIN1; UBL5
UniProt ID: (Human) Q13526
Entrez Gene ID: (Human) 5300, (Rat) 298696
positive regulation of protein phosphorylation
positive regulation of protein dephosphorylation
negative regulation of neuron apoptotic process
positive regulation of neuron apoptotic process
positive regulation of cell growth involved in cardiac muscle cell development
protein peptidyl-prolyl isomerization
cell cycle
regulation of mitotic nuclear division
neuron differentiation
negative regulation of transforming growth factor beta receptor signaling pathway
negative regulation of protein binding
regulation of cytokinesis
negative regulation of type I interferon production
negative regulation of protein catabolic process
positive regulation of GTPase activity
positive regulation of transcription from RNA polymerase II promoter
synapse organization
protein stabilization
positive regulation of ubiquitin-protein transferase activity
regulation of pathway-restricted SMAD protein phosphorylation
negative regulation of ERK1 and ERK2 cascade
positive regulation of canonical Wnt signaling pathway
regulation of protein localization to nucleus
regulation of signal transduction by p53 class mediator
negative regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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