Invitrogen

PINK1 Monoclonal Antibody (N4/15), APC

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-45655

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N4/15

Immunogen

Fusion protein amino acids 112-496 (cytoplasmic C-terminus) of human PINK1.
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

2.15mM MES/95.64mM phosphate, pH 7.4, with 0.5M EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932109

Product Specific Information

82% identical to rat and 81% identical to mouse. >30% identity with DMPK.

1 µg/mL of MA5-45655 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 50kDa.

This antibody was formerly sold as clone S4-15.

Target Information

PINK1 (PTEN-induced putative kinase 1) protects from mitochondrial dysfunction in cells by conferring different autophosphorylation activity to mitochondrial genes. The kinase domain of PINK1 appears to be critical in its protective effects. Mitochondrial proteins are critical for the regulation of cellular energy and adaptation to stress. Particularly in brain cells, mitochondrial demand is extraordinarily high, in order to accommodate aerobic and anaerobic support of high energy processes. When mitochondrial structure and/or function is disrupted in the brain, neurodegenerative diseases like Parkinson's disease are triggered.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BRPK; FLJ27236; PINK 1; PINK-1; protein kinase BRPK; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial

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Gene Aliases: 1190006F07Rik; AU042772; AW557854; BRPK; mFLJ00387; PARK6; PINK1

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UniProt ID: (Human) Q9BXM7, (Mouse) Q99MQ3

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Entrez Gene ID: (Human) 65018, (Rat) 298575, (Mouse) 68943

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Function(s)
magnesium ion binding protease binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding calcium-dependent protein kinase activity kinase activity peptidase activator activity ubiquitin protein ligase binding protein kinase B binding C3HC4-type RING finger domain binding molecular_function nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding
Process(es)
mitophagy positive regulation of protein phosphorylation regulation of oxidative phosphorylation protein phosphorylation ubiquitin-dependent protein catabolic process response to stress response to oxidative stress mitochondrion organization regulation of hydrogen peroxide metabolic process negative regulation of gene expression regulation of mitochondrion organization positive regulation of peptidase activity macroautophagy positive regulation of macroautophagy negative regulation of macroautophagy phosphorylation protein ubiquitination peptidyl-serine phosphorylation respiratory electron transport chain regulation of protein ubiquitination positive regulation of protein ubiquitination activation of protein kinase B activity positive regulation of synaptic transmission, dopaminergic positive regulation of peptidyl-serine phosphorylation positive regulation of dopamine secretion cellular response to oxidative stress positive regulation of protein dephosphorylation intracellular signal transduction peptidyl-serine autophosphorylation TORC2 signaling positive regulation of I-kappaB kinase/NF-kappaB signaling regulation of protein complex assembly negative regulation of neuron apoptotic process positive regulation of translation negative regulation of JNK cascade protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of ubiquitin-protein transferase activity regulation of mitochondrial membrane potential positive regulation of protein kinase B signaling regulation of proteasomal protein catabolic process cellular response to hypoxia establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of mitochondrial fission positive regulation of release of cytochrome c from mitochondria negative regulation of mitochondrial fission cellular response to toxic substance mitophagy in response to mitochondrial depolarization regulation of synaptic vesicle transport negative regulation of autophagosome assembly positive regulation of mitochondrial electron transport, NADH to ubiquinone regulation of mitophagy negative regulation of mitophagy negative regulation of oxidative stress-induced cell death negative regulation of oxidative stress-induced neuron death regulation of protein targeting to mitochondrion negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide positive regulation of cristae formation positive regulation of protein targeting to mitochondrion positive regulation of free ubiquitin chain polymerization regulation of reactive oxygen species metabolic process negative regulation of reactive oxygen species metabolic process positive regulation of ATP biosynthetic process biological_process positive regulation of catecholamine secretion regulation of apoptotic process regulation of neuron apoptotic process negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway autophagy
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