Proteintech

PIP4K2A Polyclonal Antibody

View all (15) PIP4K2A antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (10)

PIP4K2A Antibody in Immunocytochemistry (ICC/IF)

PIP4K2A Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

PIP4K2A Antibody (12469-1-AP) in ICC/IF

Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PIP4K2A Antibody in Immunocytochemistry (ICC/IF)

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PIP4K2A Antibody in Western Blot (WB)

PIP4K2A Antibody in Western Blot (WB)

PIP4K2A Antibody in Western Blot (WB)

PIP4K2A Antibody in Western Blot (WB)

PIP4K2A Antibody in Immunoprecipitation (IP)

Product Image Gallery

PIP4K2A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen tissue slide using 12469-1-AP ( PIP4K2A Antibody) at dilution of 1:200 (under 40x lens).

PIP4K2A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human spleen tissue slide using 12469-1-AP ( PIP4K2A Antibody) at dilution of 1:200 (under 10x lens).

PIP4K2A Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 12469-1-AP) PIP4K2A antibody at dilution of 1:3,000 incubated at room temperature for 1.5 hours.

PIP4K2A Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 12469-1-AP) PIP4K2A antibody at dilution of 1:5,000 incubated at room temperature for 1.5 hours.

PIP4K2A Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with (Product # 12469-1-AP) PIP4K2A antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

PIP4K2A Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PIP4K2A Antibody in Immunoprecipitation (IP) — IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug.

Product Details

12469-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIP4K2A Fusion Protein Ag3152 (59-406 aa encoded by BC018034)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: VMLMPDDFK AYSKIKVDNH LFNKENMPSH FKFKEYCPMV FRNLRERFGI DDQDFQNSLT RSAPLPNDSQ ARSGARFHTS YDKRYIIKTI TSEDVAEMHN ILKKYHQYIV ECHGITLLPQ FLGMYRLNVD GVEIYVIVTR NVFSHRLSVY RKYDLKGSTV AREASDKEKA KELPTLKDND FINEGQKIYI DDNNKKVFLE KLKKDVEFLA QLKLMDYSLL VGIHDVERAE QEEVECEEND GEEEGESDGT HPVGTPPDSP GNTLNSSPPL APGEFDPNID VYGIKCHENS PRKEVYFMAI IDILTHYDAK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF LDFIGHILT (59-406 aa encoded by BC018034)

Target Information

PIP4K2A lipid kinase or phosphatidylinositol-5-phosphate 4-kinase, type II, alpha, is a member of the phosphatidylinositol-5-phosphate 4-kinase family that catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. PIP4K2A plays as essential role in the phosphoinositide signal transduction cascades as the precursor to second messengers and is involved in the regulation of secretion, cell proliferation, differentiation, and motility. PIP4K2A may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic patients. Diseases associated with PIP4K2A include B-Lymphoblastic Leukemia/Lymphoma With Hyperdiploidy and Bipolar Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.

Science advances

Triscott J,Reist M,Küng L,Mosele FC,Lehner M,Gallon J,Ravi A,Arora GK,de Brot S,Lundquist M,Gallart-Ayala H,Ivanisevic J,Piscuoglio S,Cantley LC,Emerling BM,Rubin MA

Fri Feb 03 00:00:00 UTC 2023

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.

Science advances

Triscott J,Reist M,Küng L,Mosele FC,Lehner M,Gallon J,Ravi A,Arora GK,de Brot S,Lundquist M,Gallart-Ayala H,Ivanisevic J,Piscuoglio S,Cantley LC,Emerling BM,Rubin MA

Fri Feb 03 00:00:00 UTC 2023

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.

Science advances

Triscott J,Reist M,Küng L,Mosele FC,Lehner M,Gallon J,Ravi A,Arora GK,de Brot S,Lundquist M,Gallart-Ayala H,Ivanisevic J,Piscuoglio S,Cantley LC,Emerling BM,Rubin MA

Fri Feb 03 00:00:00 UTC 2023

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.

Science advances

Triscott J,Reist M,Küng L,Mosele FC,Lehner M,Gallon J,Ravi A,Arora GK,de Brot S,Lundquist M,Gallart-Ayala H,Ivanisevic J,Piscuoglio S,Cantley LC,Emerling BM,Rubin MA

Fri Feb 03 00:00:00 UTC 2023

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.

Science advances

Triscott J,Reist M,Küng L,Mosele FC,Lehner M,Gallon J,Ravi A,Arora GK,de Brot S,Lundquist M,Gallart-Ayala H,Ivanisevic J,Piscuoglio S,Cantley LC,Emerling BM,Rubin MA

Fri Feb 03 00:00:00 UTC 2023

PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition.

Science advances

Triscott J,Reist M,Küng L,Mosele FC,Lehner M,Gallon J,Ravi A,Arora GK,de Brot S,Lundquist M,Gallart-Ayala H,Ivanisevic J,Piscuoglio S,Cantley LC,Emerling BM,Rubin MA

Fri Feb 03 00:00:00 UTC 2023

A novel lncRNA lncSAMD11-1: 1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization.

The international journal of biochemistry & cell biology

Zhang M,Cai X,Liu J,Zhou J,Shi Q,Jiang Y,Kang N,Zhen X,Wu M,Qiu P,Yan G,Sun H,Li D

Sat Oct 01 00:00:00 UTC 2022

A novel lncRNA lncSAMD11-1: 1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization.

The international journal of biochemistry & cell biology

Zhang M,Cai X,Liu J,Zhou J,Shi Q,Jiang Y,Kang N,Zhen X,Wu M,Qiu P,Yan G,Sun H,Li D

Sat Oct 01 00:00:00 UTC 2022

A novel lncRNA lncSAMD11-1: 1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization.

The international journal of biochemistry & cell biology

Zhang M,Cai X,Liu J,Zhou J,Shi Q,Jiang Y,Kang N,Zhen X,Wu M,Qiu P,Yan G,Sun H,Li D

Sat Oct 01 00:00:00 UTC 2022

A novel lncRNA lncSAMD11-1: 1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization.

The international journal of biochemistry & cell biology

Zhang M,Cai X,Liu J,Zhou J,Shi Q,Jiang Y,Kang N,Zhen X,Wu M,Qiu P,Yan G,Sun H,Li D

Sat Oct 01 00:00:00 UTC 2022

PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.

Developmental cell

Ravi A,Palamiuc L,Loughran RM,Triscott J,Arora GK,Kumar A,Tieu V,Pauli C,Reist M,Lew RJ,Houlihan SL,Fellmann C,Metallo C,Rubin MA,Emerling BM

Mon Jun 07 00:00:00 UTC 2021

PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.

Developmental cell

Ravi A,Palamiuc L,Loughran RM,Triscott J,Arora GK,Kumar A,Tieu V,Pauli C,Reist M,Lew RJ,Houlihan SL,Fellmann C,Metallo C,Rubin MA,Emerling BM

Mon Jun 07 00:00:00 UTC 2021

PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.

Developmental cell

Ravi A,Palamiuc L,Loughran RM,Triscott J,Arora GK,Kumar A,Tieu V,Pauli C,Reist M,Lew RJ,Houlihan SL,Fellmann C,Metallo C,Rubin MA,Emerling BM

Mon Jun 07 00:00:00 UTC 2021

PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.

Developmental cell

Ravi A,Palamiuc L,Loughran RM,Triscott J,Arora GK,Kumar A,Tieu V,Pauli C,Reist M,Lew RJ,Houlihan SL,Fellmann C,Metallo C,Rubin MA,Emerling BM

Mon Jun 07 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; 1-phosphatidylinositol-4-phosphate kinase; 1-phosphatidylinositol-4-phosphate-5-kinase; 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha; 1-phosphatidylinositol-5-phosphate 4-kinase A; Diphosphoinositide kinase 2-alpha; Phosphatidylinositol 5-Phosphate 4-Kinase; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha; phosphatidylinositol-4-phosphate 5-kinase, type II, alpha; phosphatidylinositol-5-phosphate 4-kinase type-2 alpha; phosphatidylinositol-5-phosphate 4-kinase, type II, alpha; PI(5)P 4-kinase type II alpha; PI5P4Kalpha; PIP4KII-alpha; PIP5KIIalpha; PIP5KIII; PIPK2 alpha; PtdIns(4)P-5-kinase B isoform; PtdIns(4)P-5-kinase C isoform; PtdIns(5)P-4-kinase isoform 2-alpha; type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform

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Gene Aliases: AW742916; PI5P4KA; PIP4K2A; Pip4ka; PIP5K2; PIP5K2A; PIP5KII-alpha; PIP5KIIA; PIPK

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UniProt ID: (Human) P48426, (Mouse) O70172, (Rat) Q9R0I8

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Entrez Gene ID: (Human) 5305, (Mouse) 18718, (Rat) 116723

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ATP binding 1-phosphatidylinositol-4-phosphate 5-kinase activity 1-phosphatidylinositol-5-phosphate 4-kinase activity 1-phosphatidylinositol-3-phosphate 4-kinase activity nucleotide binding kinase activity phosphatidylinositol phosphate kinase activity transferase activity

phosphorylation megakaryocyte development phosphatidylinositol metabolic process phosphatidylinositol phosphorylation phosphatidylinositol biosynthetic process regulation of autophagy regulation of phosphatidylinositol 3-kinase signaling positive regulation of autophagosome assembly

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