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PIP5K2B Antibody (600-401-J28) in WB
Western Blot of Rabbit Anti-PIP5K2B Antibody. Lane 1: Mouse Brain homogenate samples. Load: 10 µg per lane. Primary antibody: PIP5K2B antibody at 0.2µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.
Product Details
600-401-J28
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
PIP5K2B affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B.
Conjugate
Form
Liquid
Concentration
0.65 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-PIP5K2B specific towards PIP5K2B. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, Chicken, and Primate based on 100% sequence homology. Cross-reactivity with PIP5K2B from other sources has not been determined.
Target Information
PI4K2B lipid kinase is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4K2B is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses. PI4K2B uses phosphatidylinositol as its primary substrate and has no activity on phosphatidylinositol monophosphates. PI4K2B is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation. Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4, 5-bisphosphate synthesis (Wei et al., 2002 ).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: phosphatidylinositol 4-kinase; Phosphatidylinositol 4-kinase type 2-beta; Phosphatidylinositol 4-kinase type II-beta; phosphatidylinositol 4-kinase type-II beta; PI4K2B protein; PI4KII-BETA
Gene Aliases: 2610042N09Rik; 4933409G22Rik; PI4K2B; PI4KIIB; PIK42B; RCJMB04_25j21
UniProt ID: (Chicken) Q5ZIK0, (Human) Q8TCG2, (Mouse) Q8CBQ5, (Rat) Q5XIL2
Entrez Gene ID: (Chicken) 422807, (Human) 55300, (Mouse) 67073, (Rat) 305419
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