Proteintech

PIP5K3 Polyclonal Antibody

PIP5K3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

PIP5K3 Antibody (13361-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 13361-1-AP) (PIP5K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

13361-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Dot blot (DB)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIP5K3 Fusion Protein Ag4177 (1-451 aa encoded by BC032389)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MATDDKTSP TLDSANDLPR SPTSPSHLTH FKPLTPDQDE PPFKSAYSSF VNLFRFNKER AEGGQGEQQP LSGSWTSPQL PSRTQSVRSP TPYKKQLNEE LQRRSSALGD LRACTYCRKI ALSYAHSTDS NSIGEDLNAL SDSACSVSVL DPSEPRTPVG SRKASRNIFL EDDLAWQSLI HPDSSNTPLS TRLVSVQEDA GKSPARNRSA SITNLSLDRS GSPMVPSYET SVSPQANRTY VRTETTEDER KILLDSVQLK DLWKKICHHS SGMEFQDHRY WLRTHPNCIV GKELVNWLIR NGHIATRAQA IAIGQAMVDG RWLDCVSHHD QLFRDEYALY RPLQSTEFSE TPSPDSDSVN SVEGHSEPSW FKDIKFDDSD TEQIAEEGDD NLANSASPSK RTSVSSFQST VDSDSAASIS LNVELDNVNF HIKKPSKYPH VPPHPADQKG RR (1-451 aa encoded by BC032389)

Target Information

PIKFYVE belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. The content of phosphatidylinositol 3,5-bisphosphateP2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4. In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity. For a review, see Shisheva.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PIKFYVE inhibition mitigates disease in models of diverse forms of ALS.

Cell

Hung ST,Linares GR,Chang WH,Eoh Y,Krishnan G,Mendonca S,Hong S,Shi Y,Santana M,Kueth C,Macklin-Isquierdo S,Perry S,Duhaime S,Maios C,Chang J,Perez J,Couto A,Lai J,Li Y,Alworth SV,Hendricks E,Wang Y,Zlokovic BV,Dickman DK,Parker JA,Zarnescu DC,Gao FB,Ichida JK

Thu Feb 16 00:00:00 UTC 2023

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.

eLife

Giridharan SSP,Luo G,Rivero-Rios P,Steinfeld N,Tronchere H,Singla A,Burstein E,Billadeau DD,Sutton MA,Weisman LS

Tue Jan 18 00:00:00 UTC 2022

TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation.

Molecular cell

Fitzian K,Brückner A,Brohée L,Zech R,Antoni C,Kiontke S,Gasper R,Linard Matos AL,Beel S,Wilhelm S,Gerke V,Ungermann C,Nellist M,Raunser S,Demetriades C,Oeckinghaus A,Kümmel D

Thu Jul 01 00:00:00 UTC 2021

TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.

Frontiers in oncology

Jiang J,Liu D,Xu G,Liang T,Yu C,Liao S,Chen L,Huang S,Sun X,Yi M,Zhang Z,Lu Z,Wang Z,Chen J,Chen T,Li H,Yao Y,Chen W,Guo H,Liu C,Zhan X

Fri Apr 22 00:00:00 UTC 2022

miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment.

BMC immunology

Cao J,Chen L,Zhong X,Shen Y,Gao Y,Chen Q,Zu X,Liu J

Fri Jan 17 00:00:00 UTC 2020

Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis.

Autophagy

Fernandez-Mosquera L,Yambire KF,Couto R,Pereyra L,Pabis K,Ponsford AH,Diogo CV,Stagi M,Milosevic I,Raimundo N

Sun Sep 01 00:00:00 UTC 2019

Inhibition of c-Rel DNA binding is critical for the anti-inflammatory effects of novel PIKfyve inhibitor.

European journal of pharmacology

Terajima M,Kaneko-Kobayashi Y,Nakamura N,Yuri M,Hiramoto M,Naitou M,Hattori K,Yokota H,Mizuhara H,Higashi Y

Sun Jun 05 00:00:00 UTC 2016

Inhibition of c-Rel DNA binding is critical for the anti-inflammatory effects of novel PIKfyve inhibitor.

European journal of pharmacology

Terajima M,Kaneko-Kobayashi Y,Nakamura N,Yuri M,Hiramoto M,Naitou M,Hattori K,Yokota H,Mizuhara H,Higashi Y

Sun Jun 05 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: 1-phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol-3-phosphate 5-kinase; epididymis luminal protein 37; FYVE finger-containing phosphoinositide kinase; p235; Phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; phosphatidylinositol-4-phosphate 5-kinase, type III; phosphoinositide kinase, FYVE finger containing; PIKfyve; PIPkin-III; PtdIns(4)P-5-kinase; Serine-protein kinase PIKFYVE; type III PIP kinase; zinc finger, FYVE domain containing 29

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Gene Aliases: 5230400C17Rik; CFD; FAB1; HEL37; KIAA0981; p235; PIKFYVE; PIP5K; PIP5K3; Pipk5k3; PipkIII; ZFYVE29

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UniProt ID: (Human) Q9Y2I7, (Mouse) Q9Z1T6

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Entrez Gene ID: (Human) 200576, (Rat) 316457, (Mouse) 18711

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Function(s)

1-phosphatidylinositol-3-phosphate 5-kinase activity protein binding ATP binding zinc ion binding 1-phosphatidylinositol-4-phosphate 5-kinase activity phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity phosphatidylinositol phosphate kinase activity metal ion binding nucleotide binding kinase activity transferase activity

Process(es)

receptor-mediated endocytosis myelin assembly protein localization to nucleus intracellular signal transduction retrograde transport, endosome to Golgi cellular protein metabolic process phosphatidylinositol phosphorylation phosphorylation phosphatidylinositol metabolic process phosphatidylinositol biosynthetic process phosphatidylinositol-3-phosphate biosynthetic process phosphatidylinositol 5-phosphate metabolic process regulation of autophagosome assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PIP5K3 Polyclonal Antibody

PIP5K3 Antibody (13361-1-AP) in IHC (P)

Product Details

13361-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Dot blot (DB)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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