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Invitrogen
PISD Polyclonal Antibody
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FIGURE: 1 / 1
PISD Antibody (PA5-110173) in WB
Western blot analysis of PISD in extracts of various cell lines. Samples were incubated with PISD Polyclonal antibody (Product # PA5-110173) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-110173
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human PISD (NP_055153.1).
Conjugate
Form
Liquid
Concentration
1.81 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855584
Product Specific Information
Immunogen sequence: MMCQSEARQG PELRAAKWLH FPQLALRRRL GQLSCMSRPA LKLRSWPLTV LYYLLPFGAL RPLSRVGWRP VSRVALYKSV PTRLLSRAWG RLNQVELPHW LRRPVYSLYI WTFGVNMKEA AVEDLHHYRN LSEFFRRKLK PQARPVCGLH SVISPSDGRI LNFGQVKNCE VEQVKGVTYS LESFLGPRMC TEDLPFPPAA SCDSFKNQLV TREGNELYHC VIYLAPGDYH CFHSPTDWTV SHRRHFPGSL MSVNPGMARW IKELFCHNER VVLTGDWKHG FFSLTAVGAT NVGSIRIYFD RDLHTNSPRH SKGSYNDFSF VTHTNREGVP MRKGEHLGEF NLGSTIVLIF EAPKDFNFQL KTGQKIRFGE ALGSL
Target Information
Phosphatidylserine decarboxylases catalyze the formation of phosphatidylethanolamine (PE) by decarboxylation of phosphatidylserine (PS). Type I PSDs, such as PISD, are targeted to the inner mitochondrial membrane by an N-terminal targeting sequence. PISD also contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp566G2246; phosphatidylserine decarboxylase proenzyme; Phosphatidylserine decarboxylase proenzyme, mitochondrial
Gene Aliases: 9030221M09Rik; DJ858B16; dJ858B16.2; PISD; PSD; PSDC; PSSC
UniProt ID: (Human) Q9UG56, (Mouse) Q8BSF4
Entrez Gene ID: (Human) 23761, (Mouse) 320951
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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