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PITX3 Antibody (710212) in ICC/IF
Immunofluorescent analysis of PITX3 in HeLa cells using a PITX3 Recombinant Rabbit Superclonal™ Antibody (Product # 710212) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing localization of PiTX3.
Product Details
710212
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
7M5HCLC
Immunogen
Recombinant protein corresponding to amino acids 1-65 of human PiTX3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2532628
Product Specific Information
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
This gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aphakia; Homeobox protein PITX3; MGC12766; paired-like homeodomain 3; Paired-like homeodomain transcription factor 3; Pituitary homeobox 3; Pituitary homeobox 3 (Homeobox protein PITX3)
Gene Aliases: ak; ASMD; ASOD; CTPP4; CTRCT11; PITX3; PTX3
UniProt ID: (Human) O75364, (Mouse) O35160
Entrez Gene ID: (Human) 5309, (Mouse) 18742
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific DNA binding
lens development in camera-type eye
lens morphogenesis in camera-type eye
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
locomotory behavior
organ morphogenesis
midbrain development
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
neuron development
lens fiber cell differentiation
dopaminergic neuron differentiation
transcription, DNA-templated
multicellular organism development
regulation of gene expression
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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