Proteintech

PIWIL1 Polyclonal Antibody

PIWIL1 Antibody in Immunocytochemistry (ICC/IF)

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PIWIL1 Antibody (15659-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 15659-1-AP (PIWIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PIWIL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PIWIL1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15659-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIWIL1 Fusion Protein Ag8056 (1-350 aa encoded by BC028581)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTGRARARA RGRARGQETA QLVGSTASQQ PGYIQPRPQP PPAEGELFGR GRQRGTAGGT AKSQGLQISA GFQELSLAER GGRRRDFHDL GVNTRQNLDH VKESKTGSSG IIVRLSTNHF RLTSRPQWAL YQYHIDYNPL MEARRLRSAL LFQHEDLIGK CHAFDGTILF LPKRLQQKVT EVFSKTRNGE DVRITITLTN ELPPTSPTCL QFYNIIFRRL LKIMNLQQIG RNYYNPNDPI DIPSHRLVIW PGFTTSILQY ENSIMLCTDV SHKVLRSETV LDFMFNFYHQ TEEHKFQEQV SKELIGLVVL TKYNNKTYRV DDIDWDQNPK STFKKADGSE VSFLEYYRKQ Y (1-350 aa encoded by BC028581)

Target Information

Hiwi is a member of the Piwi /Argonaute protein family. Hiwi is specifically expressed in germline cells such as spermatocytes and round spermatids during spermatogenesis. Hiwi is also expressed in CD34+ hematopoietic progenitors, but not in CD34- hematopoietic cells, suggesting a role for this gene in the maintenance of hematopoietic stem cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Amniotes co-opt intrinsic genetic instability to protect germ-line genome integrity.

Nature communications

Sun YH,Cui H,Song C,Shen JT,Zhuo X,Wang RH,Yu X,Ndamba R,Mu Q,Gu H,Wang D,Murthy GG,Li P,Liang F,Liu L,Tao Q,Wang Y,Orlowski S,Xu Q,Zhou H,Jagne J,Gokcumen O,Anthony N,Zhao X,Li XZ

Mon Feb 13 00:00:00 UTC 2023

piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.

Journal of translational medicine

Xie Q,Li Z,Luo X,Wang D,Zhou Y,Zhao J,Gao S,Yang Y,Fu W,Kong L,Sun T

Sat Jan 29 00:00:00 UTC 2022

Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice.

Andrologia

Cai YT,Xiong CL,Liu TS,Shen SL,Rao JP,Qiu F

Wed Sep 01 00:00:00 UTC 2021

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.

Development (Cambridge, England)

Wang X,Wen Y,Zhang J,Swanson G,Guo S,Cao C,Krawetz SA,Zhang Z,Yuan S

Thu Apr 01 00:00:00 UTC 2021

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.

Development (Cambridge, England)

Wang X,Wen Y,Zhang J,Swanson G,Guo S,Cao C,Krawetz SA,Zhang Z,Yuan S

Thu Apr 01 00:00:00 UTC 2021

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.

Development (Cambridge, England)

Wang X,Wen Y,Zhang J,Swanson G,Guo S,Cao C,Krawetz SA,Zhang Z,Yuan S

Thu Apr 01 00:00:00 UTC 2021

High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.

The Prostate

Baumann B,Lugli G,Gao S,Zenner M,Nonn L

Sat Jun 01 00:00:00 UTC 2019

PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis.

Molecular therapy. Nucleic acids

Shen S,Yu H,Liu X,Liu Y,Zheng J,Wang P,Gong W,Chen J,Zhao L,Xue Y

Fri Mar 02 00:00:00 UTC 2018

PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis.

Molecular therapy. Nucleic acids

Shen S,Yu H,Liu X,Liu Y,Zheng J,Wang P,Gong W,Chen J,Zhao L,Xue Y

Fri Mar 02 00:00:00 UTC 2018

Bioinformatics

Protein Aliases: CT80; HILI; mili; Miwi like; Miwi likw; piwi homolog; piwi like homolog 1; piwi-like homolog 1; Piwi-like protein 1; piwi-like protein 2; piwi-like RNA-mediated gene silencing 1; PIWIL1L; piwil2-like protein

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Gene Aliases: CT80.1; HIWI; MIWI; PIWI; PIWIL1

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UniProt ID: (Human) Q96J94, (Mouse) Q9JMB7

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Entrez Gene ID: (Human) 9271, (Mouse) 57749

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Function(s)

single-stranded RNA binding mRNA binding protein binding protein kinase binding piRNA binding nucleic acid binding RNA binding

Process(es)

regulation of translation multicellular organism development spermatid development gene silencing by RNA piRNA metabolic process meiotic cell cycle pachytene spermatogenesis cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PIWIL1 Polyclonal Antibody

PIWIL1 Antibody (15659-1-AP) in ICC/IF

Product Details

15659-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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