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PIWIL2 Antibody (PA5-47854) in WB
Western blot analysis of PIWIL2 in rat testis tissue. Samples were incubated in PIWIL2 polyclonal antibody (Product # PA5-47854) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for PIWIL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47854
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human PIWIL2 Lys216-Asp379
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606454
Product Specific Information
In direct ELISAs, less than 4% cross-reactivity with recombinant human PIWIL4 is observed.
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
The piwi gene of Drosophila belongs to a novel class of evolutionarily conserved genes (the piwi or Argonaute family). The Piwi family is required for germ and stem cell development in invertebrates, and two Piwi members, MIWI and MILI (Miwi like, Piwil2, Piwi-like protein 2), are essential for spermatogenesis in mouse. MIWI and MILI partner with Piwi-interacting RNAs (piRNAs) which are 26-31 nucleotides in length clearly distinct from the 21-23 nucleotides of microRNAs (miRNAs) or short interfering RNAs (siRNAs). A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. MILI might be programmed by distinct piRNA populations at different stages of germ cell development, suggesting MILI plays an important role in meiotic prophase progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 80kDa PIWIL2 short isoform; Cancer/testis antigen 80; CT80; Miwi like; piwi like homolog 2; PIWI-L2; piwi-like 2; Piwi-like protein 2; piwi-like RNA-mediated gene silencing 2; piwil2-like protein; testicular tissue protein Li 141
Gene Aliases: CT80; HILI; mili; PIWIL1L; PIWIL2
UniProt ID: (Human) Q8TC59
Entrez Gene ID: (Human) 55124, (Rat) 306011
RNA 5'-end processing
spermatogenesis
germ-line stem cell population maintenance
gene silencing by RNA
piRNA metabolic process
DNA methylation involved in gamete generation
positive regulation of translation
oogenesis
positive regulation of meiosis I
multicellular organism development
meiotic cell cycle
positive regulation of histone H3-K14 acetylation
positive regulation of histone H3-K9 acetylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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