Invitrogen

PKA alpha Polyclonal Antibody

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View all (18) PKA alpha antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-21842

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11151993

Product Specific Information

Recommended positive controls: HeLaS3, NIH-3T3, C2C12.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (94%), Dog (99%), Rabbit (97%), Sheep (99%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C(s); C-alpha-2; C-alpha-S; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; MGC102831; MGC48865; PKA; PKA C-alpha; pkac; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit

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Gene Aliases: PKACA; PKCD; PPNAD4; PRKACA

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UniProt ID: (Human) P17612, (Mouse) P05132

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Entrez Gene ID: (Human) 5566, (Mouse) 18747

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Function(s)
protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding protein complex binding protein kinase A regulatory subunit binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rab GTPase binding
Process(es)
G2/M transition of mitotic cell cycle mesoderm formation neural tube closure regulation of heart rate stimulatory C-type lectin receptor signaling pathway renal water homeostasis mRNA processing protein phosphorylation blood coagulation positive regulation of cell proliferation regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation activation of protein kinase A activity calcium-mediated signaling using intracellular calcium source lipoprotein metabolic process regulation of protein binding regulation of cellular respiration regulation of osteoblast differentiation protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation negative regulation of meiotic cell cycle regulation of cytosolic calcium ion concentration regulation of synaptic transmission, glutamatergic regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity regulation of proteasomal protein catabolic process regulation of protein processing positive regulation of cell cycle arrest cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning regulation of cardiac conduction regulation of bicellular tight junction assembly phosphorylation modulation of synaptic transmission
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