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          • Primary Antibodies ›
          • PKC alpha/beta/gamma Antibodies

          Bioss

          PKC alpha/beta/gamma Polyclonal Antibody

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          Datasheet
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          Cite PKC alpha/beta/gamma Polyclonal Antibody

          Product Details

          BS-3531R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Dog, Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic peptide derived from human PKC alpha.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit C alpha; PKA C-alpha; PKC-B; PKC-beta; PKC-gamma; protein kinase A; protein kinase A alpha; protein kinase A catalytic subunit; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; Protein kinase C gamma type; protein kinase C type I (gamma type); protein kinase C, beta 1; protein kinase C, beta 1 polypeptide; protein kinase C, gamma; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit

          View more View less

          Gene Aliases: A130082F03Rik; Cs-PKA; PKACA; PKC; PKC-Beta; PKC-gamma; PKCA1; PKCB; PKCC; PKCD; PKCG; PKCgamma; PKCI; PPNAD4; PRKACA; Prkc; PRKCB; PRKCB1; PRKCB2; Prkcc; PRKCG; RATPKCI; SCA14

          View more View less

          UniProt ID: (Human) P17612, (Dog) Q8MJ44, (Human) P05771, (Human) P05129, (Mouse) P05132, (Mouse) P68404, (Rat) P68403, (Mouse) P63318, (Rat) P63319

          View more View less

          Entrez Gene ID: (Human) 5566, (Dog) 403556, (Human) 5579, (Dog) 489968, (Human) 5582, (Dog) 100855828, (Rat) 25636, (Mouse) 18747, (Mouse) 18751, (Rat) 25023, (Mouse) 18752, (Rat) 24681

          View more View less

          Function(s)
          protein serine/threonine kinase activity cAMP-dependent protein kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding protein kinase binding ubiquitin protein ligase binding protein complex binding protein kinase A regulatory subunit binding chromatin binding protein kinase C activity calcium-dependent protein kinase C activity protein kinase C binding calcium channel regulator activity zinc ion binding ligand-dependent nuclear receptor transcription coactivator activity histone kinase activity (H3-T6 specific) histone binding androgen receptor binding protein kinase activity Rab GTPase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding
          Process(es)
          G2/M transition of mitotic cell cycle mesoderm formation neural tube closure regulation of heart rate stimulatory C-type lectin receptor signaling pathway renal water homeostasis mRNA processing protein phosphorylation blood coagulation positive regulation of cell proliferation regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation activation of protein kinase A activity calcium-mediated signaling using intracellular calcium source lipoprotein metabolic process regulation of protein binding regulation of cellular respiration regulation of osteoblast differentiation protein autophosphorylation positive regulation of protein export from nucleus sperm capacitation negative regulation of meiotic cell cycle regulation of cytosolic calcium ion concentration regulation of synaptic transmission, glutamatergic regulation of cardiac muscle contraction regulation of ryanodine-sensitive calcium-release channel activity regulation of proteasomal protein catabolic process regulation of protein processing positive regulation of cell cycle arrest cellular response to glucose stimulus cellular response to parathyroid hormone stimulus cellular response to glucagon stimulus cellular response to epinephrine stimulus cell communication by electrical coupling involved in cardiac conduction negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning regulation of cardiac conduction regulation of bicellular tight junction assembly chemical synaptic transmission learning or memory chemosensory behavior phosphorylation platelet activation negative regulation of protein ubiquitination regulation of response to food positive regulation of mismatch repair intracellular signal transduction negative regulation of protein catabolic process regulation of circadian rhythm response to morphine negative regulation of neuron apoptotic process response to pain rhythmic process regulation of phagocytosis innervation negative regulation of proteasomal protein catabolic process response to hypoxia immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter calcium ion transport cellular calcium ion homeostasis apoptotic process negative regulation of glucose transport regulation of dopamine secretion chromatin modification positive regulation of vascular endothelial growth factor receptor signaling pathway histone H3-T6 phosphorylation regulation of growth B cell activation response to drug positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis negative regulation of insulin receptor signaling pathway B cell receptor signaling pathway positive regulation of B cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity cellular response to carbohydrate stimulus adaptive immune response mitotic nuclear envelope disassembly signal transduction lipoprotein transport modulation of synaptic transmission response to glucose dibenzo-p-dioxin metabolic process response to vitamin D positive regulation of odontogenesis of dentin-containing tooth response to ethanol
          It has to be done as per old AB suggested Products section.
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