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PKLR Antibody (PA5-100074) in ICC/IF
Immunofluorescent analysis of PKLR in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and PKLR polyclonal antibody (Product # PA5-100074) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100074
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PKLR(Accession P30613), corresponding to amino acid residues R99-R149.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815604
Product Specific Information
Antibody detects endogenous levels of total PKLR.
Target Information
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: isozymes R/L; L-PK; Pyruvate kinase 1; pyruvate kinase isozyme R/L; Pyruvate kinase isozymes L/R; pyruvate kinase isozymes R/L; Pyruvate kinase PKLR; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and red blood cell; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
Gene Aliases: Pk-1; PK1; PKL; Pklg; PKLR; PKR; PKRL; R-PK; RPK
UniProt ID: (Human) P30613, (Rat) P12928
Entrez Gene ID: (Human) 5313, (Mouse) 18770, (Rat) 24651
carbohydrate metabolic process
glycolytic process
ATP biosynthetic process
metabolic process
response to heat
response to lithium ion
phosphorylation
cellular response to insulin stimulus
response to ATP
pyruvate biosynthetic process
response to cAMP
response to other organism
response to hypoxia
response to nutrient
response to glucose
canonical glycolysis
response to metal ion
response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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