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PKLR Antibody (PA5-34734) in WB
Western blot was performed using Anti-PKLR Polyclonal Antibody (Product # PA5-34734) and bands between 58-62 kDa band corresponding to PKLR was observed in K-562 and Hep G2, but not in U-2 OS, U-937 and Raji which are reported to be low expressing cell lines. Whole cell lysate (30 µg lysate) of K-562 (Lane 1), Hep G2 (Lane 2), U-2 OS (Lane 3), U-937 (Lane 4) and Raji (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product #... View More
Product Details
PA5-34734
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Pyruvate Kinase (liver/RBC). The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.62 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2552086
Product Specific Information
Recommended positive controls: K562, Raw264.7, rat liver, human PKLR-transfected 293T.
Predicted reactivity: Mouse (90%), Rat (90%), Dog (89%), Bovine (91%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: isozymes R/L; L-PK; Pyruvate kinase 1; pyruvate kinase isozyme R/L; Pyruvate kinase isozymes L/R; pyruvate kinase isozymes R/L; Pyruvate kinase PKLR; pyruvate kinase type L; pyruvate kinase, liver and blood cell; pyruvate kinase, liver and red blood cell; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
Gene Aliases: Pk-1; PK1; PKL; Pklg; PKLR; PKR; PKRL; R-PK; RPK
UniProt ID: (Human) P30613, (Rat) P12928
Entrez Gene ID: (Human) 5313, (Mouse) 18770, (Rat) 24651
carbohydrate metabolic process
glycolytic process
ATP biosynthetic process
metabolic process
response to heat
response to lithium ion
phosphorylation
cellular response to insulin stimulus
response to ATP
pyruvate biosynthetic process
response to cAMP
response to other organism
response to hypoxia
response to nutrient
response to glucose
canonical glycolysis
response to metal ion
response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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