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PLA2G2A Antibody (MA5-37885) in WB
Western blot analysis of PLA2G2A in extracts of various cell lines. Samples were incubated with PLA2G2A Monoclonal antibody (Product # MA5-37885) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
MA5-37885
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3X9F5
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 65-144 of human PLA2G2A (P14555)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897805
Product Specific Information
Positive Samples: HT-29, HepG2, DU145, Mouse liver, Mouse spleen, Mouse small intestine, Rat spleen, Rat small intestine, Rat liver
Immunogen sequence: VTHDCCYKRL EKRGCGTKFL SYKFSNSGSR ITCAKQDSCR SQLCECDKAA ATCFARNKTT YNKKYQYYSN KHCRGSTPRC
Target Information
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EC 3.1.1.4; EF; Enhancing factor; eted enzyme type IIA phospholipase A2; GIIC sPLA2; Group IIA phospholipase A2; modifier of Min1; non-pancreatic secreted type II phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); Phospholipase A2, membrane associated; PLA2BPLA2; PLA2LPLA2S; platelet phospholipase A2; secretory group II phospholipase A2
Gene Aliases: EF; MOM1; PLA2; PLA2B; PLA2G2A; PLA2L; PLA2S; PLAS1; RASF-A; sPLA2; sPla2-IIA
UniProt ID: (Human) P14555, (Rat) P14423, (Mouse) P31482
Entrez Gene ID: (Human) 5320, (Rat) 29692, (Mouse) 18780
phospholipid metabolic process
negative regulation of cell proliferation
lipid catabolic process
somatic stem cell population maintenance
regulation of cell proliferation
phosphatidic acid metabolic process
regulation of epithelial cell proliferation
negative regulation of epithelial cell proliferation
phosphatidic acid biosynthetic process
positive regulation of macrophage derived foam cell differentiation
low-density lipoprotein particle remodeling
phosphatidylglycerol acyl-chain remodeling
phosphatidylinositol acyl-chain remodeling
phosphatidylserine acyl-chain remodeling
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
positive regulation of inflammatory response
defense response to Gram-positive bacterium
positive regulation of ERK1 and ERK2 cascade
lipid metabolic process
regulation of growth
negative regulation of T cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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