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PLA2G5 Antibody (MA1-830) in ICC/IF
Immunofluorescence analysis of Group V secretory phospholipase A2 (PLAG5) was performed using 90% confluent log phase NCCIT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PLA2G5 (3G1) Mouse Monoclonal Antibody (Product # MA1-830) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa ... View More
Product Details
MA1-830
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3G1
Immunogen
Full length W79A human group V phospholipase A2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2164744
Product Specific Information
MA1-830 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
MA1-830 detects Group V Secretory Phospholipase A2 from human and mouse samples.
MA1-830 has been successfully used in Western blot procedures. By Western blot, MA1-830 detects a ~15 kDa band representing Group V Secretory Phospholipase A2 from mouse macrophages.
The MA1-830 immunogen is full length W79A human group V phospholipase A2.
Target Information
This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ca2+-dependent phospholipase A2; calcium-dependent phospholipase A2; DKFZp686C2294; group V phospholipase A2; MGC46205; Phosphatidylcholine 2-acylhydrolase 5; Phospholipase A2 group V; phospholipase A2, group V; PLA2-10
Gene Aliases: FRFB; GV-PLA2; hVPLA(2); PLA2; PLA2-10; PLA2G5; sPLA2
UniProt ID: (Human) P39877, (Mouse) P97391
Entrez Gene ID: (Human) 5322, (Mouse) 18784
lipid metabolic process
phospholipid metabolic process
platelet activating factor biosynthetic process
positive regulation of phospholipase activity
lipid catabolic process
leukotriene biosynthetic process
arachidonic acid secretion
positive regulation of ERK1 and ERK2 cascade
phosphatidic acid biosynthetic process
response to cytokine
phosphatidylglycerol acyl-chain remodeling
phosphatidylinositol acyl-chain remodeling
phosphatidylserine acyl-chain remodeling
phosphatidylcholine acyl-chain remodeling
phosphatidylethanolamine acyl-chain remodeling
response to cAMP
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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